AT107

Annexin V-APC/PI Apoptosis Kit (贴壁细胞专用)

¥1,010.00 – ¥2,090.00

因产品会迭代升级，具体实验步骤请按纸质版说明书操作

说明书

在售SKU:70-AT107-100, 70-AT107-60, 70-AT107-30, 凋亡, 细胞凋亡, 凋亡试剂盒, 细胞凋亡试剂盒, 凋亡检测, 细胞凋亡检测, 凋亡检测试剂盒, 细胞凋亡检测试剂盒, Annexin V, Annexin, Apoptosis, 贴壁, 贴壁细胞

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库存号

文章目录[隐藏]

产品简介
产品特点
结果示例
试剂盒组分

产品简介

Annexin V(或 Annexin A5)为胞内蛋白膜联蛋白家族成员，以钙依赖的方式与磷脂酰丝氨酸(PS)结合。PS存在于正常细胞浆膜的内层，但在凋亡早期，膜不对称性丧失，PS 易位至细胞表面。荧光标记的 Annexin V可与之特异性结合，表明该细胞为凋亡细胞。
PI(碘化丙啶)是一种核酸染料，它不能透过正常细胞或早期凋亡细胞的完整的细胞膜，但可以透过凋亡晚期和坏死细胞的细胞膜而使细胞核染红。因此，将 Annexin V 与 PI 联合使用时，PI 则被排除在活细胞(Annexin V-/PI-)和早期凋亡细胞(Annexin V+/PI-)之外，而晚期凋亡细胞和坏死细胞同时被荧光标记的Annexin V 和 PI 结合染色呈现双阳性(Annexin V+/PI+)。
Accutase 是一种天然的酶，兼具蛋白水解酶和胶原酶的活性。但它比胰酶和胶原酶更有效，使用浓度更低，使之毒性更小、更温和。因此对细胞的损伤更小，可减少因消化处理带来的假阳性。即便长时间消化，也可保持 90%以上的细胞活力。适合于各种贴壁细胞、胚胎和神经元干细胞等较敏感的细胞的消化。
本试剂盒仅需 15 分钟即可染色样本，快速方便。提供阳性质控液，便于制备单染管，调节补偿。提供消化细胞更温和的 Accutase，减少假阳性。即便长时间消化，也可保持 90%以上的细胞活力。适合于各种贴壁细胞、胚胎和神经元干细胞等较敏感的细胞的消化。本试剂盒仅需 15 分钟即可染色样本，快速方便。提供阳性质控液，便于制备单染管，调节补偿。提供消化细胞更温和的 Accutase，减少假阳性。