EK180EG

EasyGo!™ Human IFN-γ One-Step ELISA Kit检测试剂盒(酶联免疫吸附法)

因产品会迭代升级,具体实验步骤请按纸质版说明书操作

在售SKU:70-EK180EG-48, 70-EK180EG-96, EK180EG

  • 分子靶点:IFNG
  • 种属:
  • 样本类型:血清,血浆,细胞培养上清及其他生物学样本
  • 检测样本体积:20μL
  • 灵敏度:6.59pg/mL
  • 检测范围:23.44-1500pg/mL
  • 回收率:70%-130%
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ELISA试剂盒详细信息

商品名EasyGo!™ Human IFN-γ One-Step ELISA Kit检测试剂盒(酶联免疫吸附法)
种属
靶点IFNG
检测方法双抗体夹心法
检测样本类型血清,血浆,细胞培养上清及其他生物学样本
检测样本体积20μL
灵敏度6.59pg/mL
线性范围23.44-1500pg/mL
精密度板内变异系数:< 10%;板间变异系数:< 10%
回收率70%-130%
板式96孔板,可拆
保存条件2-8℃
运输条件2-8℃冰袋运输
检测原理本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性捕获抗体预包被在高亲和力的酶标板上,酶标板孔中预先加入辣根过氧化物酶标记的检测抗体微球。酶标板孔中加入标准品和待测样本,经过孵育,样本中存在的待测物质与捕获抗体和检测抗体结合。洗涤去除未结合的物质后,加入显色底物(TMB),避光显色。颜色反应的深浅与样本中待测物质的浓度成正比。加入终止液终止反应,在450nm波长 (参考波长570 - 630nm) 测定吸光度值。

分子信息

概述人 IFNG, IFN-γ 靶点信息

IFNG 分子靶点信息概述

  • 分子名:IFNG, interferon gamma
  • 基因家族:Interferons

IFNG 分子靶点综述

IFN-γ主要由Th1型细胞产生,属于II型细胞因子中干扰素家族中的一员,是一类分泌型糖蛋白,由143个氨基酸组成,相对分子质量是16942。IFN-γ主要由活化的T细胞和NK细胞产生,具有调节机体免疫状态,介导细胞免疫,加强受体介导的吞噬功能,增强巨噬细胞和中性粒细胞的杀伤活性,具有抗病毒感染及抗肿瘤等生物学活性。

人 Human IFNG 分子靶点信息

  • 分子名:IFNG, interferon gamma
  • 别称:
    • IFG
    • IFI
    • IFN-gamma
    • IMD69
    • immune interferon
    • interferon, gamma
  • 基因序列:NCBI_Gene: 3458
  • 蛋白序列:UniProtKB: P01579

人 Human IFNG靶点分子功能(预测)

Predicted to enable cytokine activity. Involved in several processes, including positive regulation of cell death; positive regulation of cell differentiation; and positive regulation of macromolecule metabolic process. Acts upstream of or within several processes, including positive regulation of gene expression; positive regulation of protein phosphorylation; and response to virus. Located in extracellular region. Implicated in several diseases, including ataxia telangiectasia; autoimmune disease (multiple); eczema herpeticum; factor VIII deficiency; and leukemia (multiple). Biomarker of several diseases, including acute retinal necrosis syndrome; allergic rhinitis; autoimmune disease (multiple); nephritis (multiple); and otitis media (multiple).

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