EK1258

Human Apolipoprotein AII/Apo A2 ELISA Kit检测试剂盒（酶联免疫吸附法）

¥1,600.00 – ¥2,650.00

因产品会迭代升级，具体实验步骤请按纸质版说明书操作

说明书

在售SKU:70-EK1258-48, 70-EK1258-96, EK1258

分子靶点：APOA2
种属：人
样本类型：血清、血浆、细胞培养上清及其他生物学样本
检测样本体积：血清、血浆样本10μL
灵敏度：0.53pg/mL
检测范围：7.81-500pg/mL
回收率：91%-123%

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Size
库存号

ELISA试剂盒详细信息

商品名	Human Apolipoprotein AII/Apo A2 ELISA Kit检测试剂盒（酶联免疫吸附法）
种属	人
靶点	APOA2
检测方法	双抗体夹心法
检测样本类型	血清、血浆、细胞培养上清及其他生物学样本
检测样本体积	血清、血浆样本10μL
灵敏度	0.53pg/mL
线性范围	7.81-500pg/mL
精密度	板内变异系数：3.8%-4.5%;板间变异系数：4.3%-9.4%
回收率	91%-123%
平均回收率	106%
板式	96孔板，可拆
保存条件	2-8℃保存。已拆开：标准品-20℃保存，其它4℃。
运输条件	2-8℃冰袋运输
检测原理	本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人ApoA2抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体，经过孵育，样本中存在的ApoA2与固相抗体和检测抗体结合。洗涤去除未结合的物质后，加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后，加入显色底物TMB，避光显色。颜色反应的深浅与样本中ApoA2的浓度成正比。加入终止液终止反应，在450nm波长(参考波长570 - 630nm)测定吸光度值。

文章目录[隐藏]

分子信息
APOA2 分子靶点信息概述
APOA2 分子靶点综述
- 人 Human APOA2 分子靶点信息
- 人 Human APOA2靶点分子功能（预测）

分子信息

概述人 APOA2, apolipoprotein A2 靶点信息

APOA2 分子靶点信息概述

分子名：APOA2, apolipoprotein A2
基因家族：Apolipoproteins
全称：apolipoprotein A-II

APOA2 分子靶点综述

载脂蛋白AII (Apo A2)是人血浆高密度脂蛋白(HDL)中含量仅次于Apo A1的第二大载脂蛋白，是可交换载脂蛋白中亲水性最强的。Apo A2由肝和小肠合成，以单体或者二聚体形式存在于血浆中。Apo A2基因由4个外显子和3个内含子组成。四个外显子编码5'端非翻译区、前肽、一个短N末端结构域和由可变数量的脂质结合双亲性螺旋构成的C末端结构域。 Apo A2可通过结合脂质来稳定HDL结构，并影响HDL代谢。Apo A2在脂质代谢和动脉粥样硬化中的作用展现了重要临床意义。Apo A2水平与动脉粥样硬化易感性、游离脂肪酸水平、体脂和胰岛素抵抗力的增加有关。

人 Human APOA2 分子靶点信息

分子名：APOA2, apolipoprotein A2
别称：
- Apo-AII
- ApoA-II
- apoAII
- apolipoprotein A-II
基因序列：NCBI_Gene: 336
蛋白序列：UniProtKB: P02652

人 Human APOA2靶点分子功能（预测）

Enables several functions, including cholesterol binding activity; protein dimerization activity; and signaling receptor binding activity. Contributes to cholesterol transfer activity. Involved in several processes, including lipid transport; plasma lipoprotein particle organization; and regulation of lipid metabolic process. Located in blood microparticle and extracellular exosome. Part of chylomicron; spherical high-density lipoprotein particle; and very-low-density lipoprotein particle. Implicated in familial hypercholesterolemia. Biomarker of coronary artery disease; familial combined hyperlipidemia; obesity; type 1 diabetes mellitus; and type 2 diabetes mellitus.