EK1241

Human CTLA-4/CD152 ELISA Kit检测试剂盒（酶联免疫吸附法）

¥1,600.00 – ¥2,650.00

因产品会迭代升级，具体实验步骤请按纸质版说明书操作

说明书

在售SKU:70-EK1241-48, 70-EK1241-96, EK1241

分子靶点：CTLA4
种属：人
样本类型：血清、血浆、细胞培养上清及其他生物学样本
检测样本体积：血清、血浆样本50μL
灵敏度：0.71pg/mL
检测范围：93.75-6000pg/mL
回收率：78%-120%

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Size
库存号

ELISA试剂盒详细信息

商品名	Human CTLA-4/CD152 ELISA Kit检测试剂盒（酶联免疫吸附法）
种属	人
靶点	CTLA4
检测方法	双抗体夹心法
检测样本类型	血清、血浆、细胞培养上清及其他生物学样本
检测样本体积	血清、血浆样本50μL
灵敏度	0.71pg/mL
线性范围	93.75-6000pg/mL
精密度	板内变异系数：2.4%-3.7%;板间变异系数：3.1%-4.3%
回收率	78%-120%
平均回收率	94%
板式	96孔板，可拆
保存条件	2-8℃保存。已拆开：标准品-20℃保存，其它4℃。
运输条件	2-8℃冰袋运输
检测原理	本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人CTLA-4抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、检测样本和辣根过氧化物酶标记的检测抗体，经过孵育，样本中存在的CTLA-4与固相抗体结合和检测抗体结合。洗涤后，加入显色底物TMB，避光显色。颜色反应的深浅与样本中CTLA-4的浓度成正比。加入终止液终止反应，在450nm波长(参考波长570 - 630nm)测定吸光度值。

文章目录[隐藏]

分子信息
CTLA4 分子靶点信息概述
CTLA4 分子靶点综述
- 人 Human CTLA4 分子靶点信息
- 人 Human CTLA4靶点分子功能（预测）

分子信息

概述人 CTLA4, CD152, GSE 靶点信息

CTLA4 分子靶点信息概述

分子名：CTLA4, cytotoxic T-lymphocyte associated protein 4
基因家族：CD molecules; V-set domain containing
别名：CD152; CD; GSE; CTLA-4
曾用名：CELIAC3; IDDM12
全称：celiac disease 3; insulin-dependent diabetes mellitus 12

CTLA4 分子靶点综述

细胞毒性 T 淋巴细胞相关蛋白 4 (CTLA-4)，又名 CD152，是免疫球蛋白超家族中的成员，在辅助型 T 细胞表面表达。 CTLA-4 类似于 T 细胞的共刺激蛋白 CD28，它们都结合 CD80 和 CD86。CTLA-4 传递抑制信号给 T 细胞，而 CD28 则传递刺激信号。CTLA-4 可作为免疫检查点发挥功能、下调免疫系统、当结合抗原递呈细胞表面的 CD80 或 CD86 时作为“关闭”开关。细胞内的 CTLA-4 同样存在于调节性 T 细胞中，对它们的功能发挥是重要的。该基因突变与胰岛素依赖型糖尿病、甲状腺机能亢进、桥本甲状腺炎、乳糜泻、系统性红斑狼疮、甲状腺相关眼病、原发性胆汁性肝硬化和其它自身免疫性疾病有关。CTLA-4 基因的多态性与自身免疫性疾病如自身免疫性甲状腺疾病和多发性硬化症有关，但这种关联性通常较弱。在系统性红斑狼疮中，人们发现可溶性 CTLA-4 的剪接变异体异常产生，且存在于患有该病的患者血清中。

人 Human CTLA4 分子靶点信息

分子名：CTLA4, cytotoxic T-lymphocyte associated protein 4
别称：
- ALPS5
- CD
- CD152
- cd152 antigen
- CD152 isoform
- celiac disease 3
- CELIAC3
- CTLA-4
- cytotoxic T lymphocyte associated antigen 4 short spliced form
- cytotoxic T-lymphocyte antigen 4
- cytotoxic T-lymphocyte protein 4
- cytotoxic T-lymphocyte-associated antigen 4
- cytotoxic T-lymphocyte-associated protein 4
- cytotoxic T-lymphocyte-associated serine esterase-4
- GRD4
- GSE
- ICOS
- IDDM12
- insulin-dependent diabetes mellitus 12
- ligand and transmembrane spliced cytotoxic T lymphocyte associated antigen 4
基因序列：NCBI_Gene: 1493
蛋白序列：UniProtKB: P16410

人 Human CTLA4靶点分子功能（预测）

Involved in several processes, including B cell receptor signaling pathway; negative regulation of lymphocyte activation; and positive regulation of apoptotic process. Located in several cellular components, including clathrin-coated endocytic vesicle; external side of plasma membrane; and perinuclear region of cytoplasm. Is integral component of plasma membrane. Part of protein complex involved in cell adhesion. Implicated in several diseases, including carcinoma (multiple); hematologic cancer (multiple); hepatitis B; kidney disease (multiple); and primary immunodeficiency disease (multiple). Biomarker of several diseases, including Sjogren's syndrome; anogenital venereal wart; hepatitis A; lung disease (multiple); and tropical spastic paraparesis.