EK193

Human EGF ELISA Kit检测试剂盒（酶联免疫吸附法）

¥1,600.00 – ¥2,650.00

因产品会迭代升级，具体实验步骤请按纸质版说明书操作

说明书

在售SKU:70-EK193-48, 70-EK193-96, EK193

分子靶点：EGF
种属：人
样本类型：血清、血浆、细胞培养上清及其他生物学样本
检测样本体积：血清血浆：20μL；细胞培养上清：100μL
灵敏度：0.41pg/mL
检测范围：7.81-500pg/mL
回收率：71%-106%

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Size
库存号

ELISA试剂盒详细信息

商品名	Human EGF ELISA Kit检测试剂盒（酶联免疫吸附法）
种属	人
靶点	EGF
检测方法	双抗体夹心法
检测样本类型	血清、血浆、细胞培养上清及其他生物学样本
检测样本体积	血清血浆：20μL；细胞培养上清：100μL
灵敏度	0.41pg/mL
线性范围	7.81-500pg/mL
精密度	板内变异系数：1.1%-3.7%;板间变异系数：3.2%-6.9%
回收率	71%-106%
平均回收率	91%
板式	96孔板，可拆
保存条件	2-8℃保存。已拆开：标准品-20℃保存，其它4℃。
运输条件	2-8℃冰袋运输
检测原理	本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人EGF抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体，经过孵育，样本中存在的EGF与固相抗体和检测抗体结合。洗涤去除未结合的物质后，加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后，加入显色底物TMB，避光显色。颜色反应的深浅与样本中EGF的浓度成正比。加入终止液终止反应，在450nm波长(参考波长570 - 630nm)测定吸光度值。

文章目录[隐藏]

分子信息
EGF 分子靶点信息概述
EGF 分子靶点综述
- 人 Human EGF 分子靶点信息
- 人 Human EGF靶点分子功能（预测）

分子信息

概述人 EGF, epidermal growth factor 靶点信息

EGF 分子靶点信息概述

分子名：EGF, epidermal growth factor
全称：Pro-epidermal growth factor; epidermal growth factor (beta-urogastrone)

EGF 分子靶点综述

表皮生长因子(EGF)是一种通过与其受体EGFR结合，刺激细胞生长、增殖和分化的生长因子。EGF是小分子量多肽，最迟从小鼠下颌下腺纯化得到，随后在很多人的组织中均有发现，包括下颌下腺和腮腺。由饮食中的无机碘调控的唾液EGF在维持奥罗食管和胃组织健全方面具有重要的生理作用。唾液EGF的生理作用包括治愈口腔和胃溃疡、抑制胃酸分泌、刺激DNA合成以及保护粘膜免受腔内有害因素(如胃酸、胆汁酸、胃蛋白酶和胰蛋白酶)和物理、化学和细菌因素破坏。在某些通常与受体的突变和功能异常(如不依赖于EGF水平或与EGF结合的组成性受体信号传导)有关的癌症中，可观察到EGF受体活性增强。

人 Human EGF 分子靶点信息

分子名：EGF, epidermal growth factor
别称：
- beta-urogastrone
- HOMG4
- pro-epidermal growth factor
- URG
基因序列：NCBI_Gene: 1950
蛋白序列：UniProtKB: P01133

人 Human EGF靶点分子功能（预测）

Enables growth factor activity and guanyl-nucleotide exchange factor activity. Involved in several processes, including ERK1 and ERK2 cascade; positive regulation of macromolecule metabolic process; and positive regulation of signal transduction. Acts upstream of or within positive regulation of cell population proliferation. Located in extracellular exosome. Implicated in several diseases, including Zollinger-Ellison syndrome; hepatobiliary system cancer (multiple); high grade glioma (multiple); lung non-small cell carcinoma (multiple); and primary hypomagnesemia (multiple). Biomarker of several diseases, including acute kidney failure; lupus nephritis; neurodegenerative disease (multiple); pancreatic cancer (multiple); and pancreatitis.