EK1126

Human Galectin-3 ELISA Kit检测试剂盒（酶联免疫吸附法）

¥1,600.00 – ¥2,650.00

因产品会迭代升级，具体实验步骤请按纸质版说明书操作

说明书

在售SKU:70-EK1126-48, 70-EK1126-96, EK1126

分子靶点：LGALS3
种属：人
样本类型：血清、血浆、细胞培养上清及其他生物学样本
检测样本体积：血清、血浆样本10μL
灵敏度：2.46pg/mL
检测范围：62.5-4000pg/mL
回收率：85%-105%

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Size
库存号

ELISA试剂盒详细信息

商品名	Human Galectin-3 ELISA Kit检测试剂盒（酶联免疫吸附法）
种属	人
靶点	LGALS3
检测方法	双抗体夹心法
检测样本类型	血清、血浆、细胞培养上清及其他生物学样本
检测样本体积	血清、血浆样本10μL
灵敏度	2.46pg/mL
线性范围	62.5-4000pg/mL
精密度	板内变异系数：1.7%-3.5%;板间变异系数：3.9%-4.9%
回收率	85%-105%
平均回收率	91%
板式	96孔板，可拆
保存条件	2-8℃保存。已拆开：标准品-20℃保存，其它4℃。
运输条件	2-8℃冰袋运输
检测原理	本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人Galectin-3抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体，经过孵育，样本中存在的Galectin-3与固相抗体和检测抗体结合。洗涤去除未结合的物质后，加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后，加入显色底物TMB，避光显色。颜色反应的深浅与样本中Galectin-3的浓度成正比。加入终止液终止反应，在450nm波长(参考波长570 - 630nm)测定吸光度值。

文章目录[隐藏]

分子信息
LGALS3 分子靶点信息概述
LGALS3 分子靶点综述
- 人 Human LGALS3 分子靶点信息
- 人 Human LGALS3靶点分子功能（预测）

分子信息

概述人 LGALS3, MAC-2, LGALS2 靶点信息

LGALS3 分子靶点信息概述

分子名：LGALS3, galectin 3
基因家族：Receptor ligands; Galectins
别名：MAC-2; GALIG
曾用名：LGALS2
全称：advanced glycation end-product receptor 3; lectin, galactoside-binding, soluble, 3

LGALS3 分子靶点综述

半乳糖凝集素-3(Galectin-3)属于凝集素家族，表达于胞核、胞质、线粒体、细胞表面和胞外。Galectin-3对β-半乳糖苷酶具有亲和力，并展示其抗细菌和真菌的活性。它参与很多生物学过程，如细胞黏附、细胞活化和趋化作用、细胞生长和分化、细胞周期以及凋亡等。基于其广泛的生物学功能，Galectin-3被认为与癌症、炎症和纤维化、心脏疾病、中风等有关。Galectin-3表达水平与各种类型的纤维化有关联。在肝脏纤维化、肾脏纤维化以及特发性肺纤维化(IPF)中，Galectin-3的表达上调。在急性失代偿性心力衰竭和慢性心力衰竭人群中，高表达的Galectin-3会导致更高的死亡风险。同时，Galectin-3被认为在肿瘤转移中具有重要作用。

人 Human LGALS3 分子靶点信息

分子名：LGALS3, galectin 3
别称：
- 35 kDa lectin
- advanced glycation end-product receptor 3
- carbohydrate-binding protein 35
- CBP35
- GAL3
- galactose-specific lectin 3
- GALBP
- galectin-3
- GALIG
- IgE-binding protein
- L31
- laminin-binding protein
- lectin L-29
- lectin, galactose binding, soluble 3
- lectin, galactoside binding soluble 3
- lectin, galactoside-binding, soluble, 3
- LGALS2
- MAC-2 antigen
- MAC2
基因序列：NCBI_Gene: 3958
蛋白序列：UniProtKB: P17931

人 Human LGALS3靶点分子功能（预测）

Enables several functions, including IgE binding activity; chemoattractant activity; and laminin binding activity. Involved in several processes, including leukocyte chemotaxis; positive regulation of protein localization to plasma membrane; and regulation of apoptotic process. Located in immunological synapse; mitochondrial inner membrane; and nucleus. Is active in extracellular space. Colocalizes with collagen-containing extracellular matrix. Implicated in asthma. Biomarker of aortic valve stenosis and liver cirrhosis.