EK169HS

Human G-CSF High Sensitivity ELISA Kit检测试剂盒（酶联免疫吸附法）

¥2,000.00 – ¥3,400.00

因产品会迭代升级，具体实验步骤请按纸质版说明书操作

说明书

在售SKU:70-EK169HS-48, 70-EK169HS-96

分子靶点：CSF3
种属：人
样本类型：血清、血浆、细胞培养上清及其他生物学样本
检测样本体积：血清血浆：50μL；细胞培养上清：100μL
灵敏度：0.19pg/mL
检测范围：15.63-1000pg/mL
回收率：94%-119%

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Size
库存号

ELISA试剂盒详细信息

商品名	Human G-CSF High Sensitivity ELISA Kit检测试剂盒（酶联免疫吸附法）
种属	人
靶点	CSF3
检测方法	双抗体夹心法
检测样本类型	血清、血浆、细胞培养上清及其他生物学样本
检测样本体积	血清血浆：50μL；细胞培养上清：100μL
灵敏度	0.19pg/mL
线性范围	15.63-1000pg/mL
精密度	板内变异系数：4.1%-4.8%;板间变异系数：4.4%-4.9%
回收率	94%-119%
平均回收率	106%
板式	96孔板，可拆
保存条件	2-8℃保存。已拆开：标准品-20℃保存，其它4℃。
运输条件	2-8℃冰袋运输
检测原理	本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人G-CSF抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品和待测样本，经过孵育，样本中存在的G-CSF与固相抗体结合。洗涤去除未结合的物质后，加入生物素化的检测抗体孵育。洗涤去除未结合的生物素化的抗体，加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后，加入信号增强剂孵育，洗涤去除未结合的物质后，再次加入Streptavidin-HRP。洗涤后，加入显色底物TMB，避光显色。颜色反应的深浅与样本中G-CSF的浓度成正比。加入终止液终止反应，在450nm波长(参考波长570 - 630nm)测定吸光度值。

文章目录[隐藏]

分子信息
CSF3 分子靶点信息概述
CSF3 分子靶点综述
- 人 Human CSF3 分子靶点信息
- 人 Human CSF3靶点分子功能（预测）

分子信息

概述人 CSF3, G-CSF 靶点信息

CSF3 分子靶点信息概述

分子名：CSF3, colony stimulating factor 3
基因家族：Receptor ligands
别名：MGC45931
曾用名：GCSF; G-CSF; C17orf33
全称：granulocyte colony stimulating factor; pluripoietin; filgrastim; lenograstim; chromosome 17 open reading frame 33; colony stimulating factor 3 (granulocyte)

CSF3 分子靶点综述

粒细胞集落刺激因子(G-CSF)是一种糖蛋白，可刺激骨髓产生粒细胞和干细胞，并把它们释放到血液中。从功能上来说，它是一种细胞因子和激素，是一种集落刺激因子，由内皮细胞、巨噬细胞和许多其它免疫细胞产生。G-CSF刺激中性粒细胞前体和成熟中性粒细胞的生存、增殖、分化及功能发挥。在肿瘤学和血液学上，重组G-CSF用于加速癌症患者化疗后引起的嗜中性白血球减少症的恢复。在白细胞去除前，G-CSF也可用来增加献血者血液中的造血干细胞数量，用于造血干细胞移植。

人 Human CSF3 分子靶点信息

分子名：CSF3, colony stimulating factor 3
别称：
- C17orf33
- chromosome 17 open reading frame 33
- colony stimulating factor 3 (granulocyte)
- CSF3OS
- filgrastim
- G-CSF
- GCSF
- granulocyte colony-stimulating factor
- granulocyte-colony stimulating factor
- lenograstim
- MGC45931
- pluripoietin
基因序列：NCBI_Gene: 1440
蛋白序列：UniProtKB: P09919

人 Human CSF3靶点分子功能（预测）

Enables cytokine activity and enzyme binding activity. Involved in several processes, including positive regulation of cytoskeleton organization; positive regulation of intracellular signal transduction; and positive regulation of protein phosphorylation. Predicted to be located in endocytic vesicle lumen; extracellular region; and lysosomal lumen. Predicted to be active in extracellular space. Implicated in several diseases, including allergic cutaneous vasculitis; artery disease (multiple); ischemia (multiple); leukemia (multiple); and lung disease (multiple). Biomarker of COVID-19; bacterial pneumonia; and myeloid leukemia.