EK1H01

Human HGF ELISA Kit检测试剂盒（酶联免疫吸附法）

¥1,600.00 – ¥2,650.00

因产品会迭代升级，具体实验步骤请按纸质版说明书操作

说明书

在售SKU:70-EK1H01-48, 70-EK1H01-96, EK1H01

分子靶点：HGF
种属：人
样本类型：血清、血浆、细胞培养上清及其他生物学样本
检测样本体积：血清、血浆样本20μL
灵敏度：1.99pg/mL
检测范围：62.5-4000pg/mL
回收率：85%-108%

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Size
库存号

ELISA试剂盒详细信息

商品名	Human HGF ELISA Kit检测试剂盒（酶联免疫吸附法）
种属	人
靶点	HGF
检测方法	双抗体夹心法
检测样本类型	血清、血浆、细胞培养上清及其他生物学样本
检测样本体积	血清、血浆样本20μL
灵敏度	1.99pg/mL
线性范围	62.5-4000pg/mL
精密度	板内变异系数：4.3%-6.5%;板间变异系数：3.1%-8.3%
回收率	85%-108%
平均回收率	95%
板式	96孔板，可拆
保存条件	2-8℃保存。已拆开：标准品-20℃保存，其它4℃。
运输条件	2-8℃冰袋运输
检测原理	本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人HGF抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体，经过孵育，样本中存在的HGF与固相抗体和检测抗体结合。洗涤去除未结合的物质后，加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后，加入显色底物TMB，避光显色。颜色反应的深浅与样本中HGF的浓度成正比。加入终止液终止反应，在450nm波长(参考波长570 - 630nm)测定吸光度值。

文章目录[隐藏]

分子信息
HGF 分子靶点信息概述
HGF 分子靶点综述
- 人 Human HGF 分子靶点信息
- 人 Human HGF靶点分子功能（预测）

分子信息

概述人 HGF, SF, F-TCF 靶点信息

HGF 分子靶点信息概述

分子名：HGF, hepatocyte growth factor
别名：SF; F-TCF; HGFB; HPTA
曾用名：DFNB39
全称：hepatopoietin A; fibroblast-derived tumor cytotoxic factor; scatter factor; lung fibroblast-derived mitogen; deafness, autosomal recessive 39; hepatocyte growth factor (hepapoietin A; scatter factor)

HGF 分子靶点综述

肝细胞生长因子(HGF)是旁分泌性细胞生长、运动和形态发生的因子，由间充质细胞分泌产生，可作为大部分上皮来源细胞的多功能细胞因子。HGF以单链形式存在，是一个带有29个氨基酸信号序列和25个氨基酸前体序列的728个氨基酸残基前肽。HGF结合原癌基因c-Met受体后就会激活酪氨酸激酶信号级联，进而调节细胞的生长、运动和形态发生。它在血管生成、肿瘤发生和组织再生中起核心作用。目前正在测试人HGF质粒DNA治疗心肌细胞是否可作为冠状动脉疾病和心肌梗死后心脏损伤的一种潜在治疗。HGF也可作为基孔肯雅病毒诱导的关节痛的预后指示。高水平的HGF与与快速康复有关。

人 Human HGF 分子靶点信息

分子名：HGF, hepatocyte growth factor
别称：
- deafness, autosomal recessive 39
- DFNB39
- F-TCF
- fibroblast-derived tumor cytotoxic factor
- hepatocyte growth factor (hepapoietin A, scatter factor)
- hepatopoeitin-A
- hepatopoietin-A
- HGFB
- HPTA
- lung fibroblast-derived mitogen
- SF
基因序列：NCBI_Gene: 3082
蛋白序列：UniProtKB: P14210

人 Human HGF靶点分子功能（预测）

Enables chemoattractant activity and identical protein binding activity. Involved in several processes, including cellular response to hepatocyte growth factor stimulus; negative regulation of programmed cell death; and positive regulation of nitrogen compound metabolic process. Acts upstream of or within regulation of branching involved in salivary gland morphogenesis by mesenchymal-epithelial signaling. Located in membrane. Implicated in several diseases, including artery disease (multiple); autosomal recessive nonsyndromic deafness 39; eye disease (multiple); neuropathy (multiple); and scleroderma (multiple). Biomarker of several diseases, including artery disease (multiple); biliary tract disease (multiple); myositis (multiple); retinal disease (multiple); and rheumatoid arthritis (multiple).