EK1168

Human IGFBP-3 ELISA Kit检测试剂盒（酶联免疫吸附法）

¥1,600.00 – ¥2,650.00

因产品会迭代升级，具体实验步骤请按纸质版说明书操作

说明书

在售SKU:70-EK1168-48, 70-EK1168-96, EK1168

分子靶点：IGFBP3
种属：人
样本类型：血清、血浆、细胞培养上清及其他生物学样本
检测样本体积：血清、血浆样本5μL
灵敏度：2.16pg/mL
检测范围：125-8000pg/mL
回收率：90%-111%

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Size
库存号

ELISA试剂盒详细信息

商品名	Human IGFBP-3 ELISA Kit检测试剂盒（酶联免疫吸附法）
种属	人
靶点	IGFBP3
检测方法	双抗体夹心法
检测样本类型	血清、血浆、细胞培养上清及其他生物学样本
检测样本体积	血清、血浆样本5μL
灵敏度	2.16pg/mL
线性范围	125-8000pg/mL
精密度	板内变异系数：3.9%-5.1%;板间变异系数：6.5%-7.2%
回收率	90%-111%
平均回收率	102%
板式	96孔板，可拆
保存条件	2-8℃保存。已拆开：标准品-20℃保存，其它4℃。
运输条件	2-8℃冰袋运输
检测原理	本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人IGFBP-3抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体，经过孵育，样本中存在的IGFBP-3与固相抗体和检测抗体结合。洗涤去除未结合的物质后，加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后，加入显色底物TMB，避光显色。颜色反应的深浅与样本中IGFBP-3的浓度成正比。加入终止液终止反应，在450nm波长(参考波长570 - 630nm)测定吸光度值。

文章目录[隐藏]

分子信息
IGFBP3 分子靶点信息概述
IGFBP3 分子靶点综述
- 人 Human IGFBP3 分子靶点信息
- 人 Human IGFBP3靶点分子功能（预测）

分子信息

概述人 IGFBP3, IBP3, BP53 靶点信息

IGFBP3 分子靶点信息概述

分子名：IGFBP3, insulin like growth factor binding protein 3
基因家族：Insulin like growth factor binding proteins
别名：IBP3; BP-53
全称：growth hormone-dependent binding protein; acid stable subunit of the 140 K IGF complex; binding protein 53; binding protein 29; IGF-binding protein 3; insulin-like growth factor binding protein 3

IGFBP3 分子靶点综述

胰岛素样生长因子结合蛋白3(IGFBP-3)，在人类中由IGFBP3基因编码。IGFBP-3是六个IGF结合蛋白(IGFBP-1至6)中的一个，这些结合蛋白具有高度保守结构，并可高亲和力结合IGF-1和IGF-2。IGFBP-3是血液中IGF的主要转运蛋白。它与细胞表面蛋白相互作用，影响胞外或内化后的细胞信号，它也可进入细胞核，在这里，它结合核激素受体和其它配体。基于细胞生长实验、动物癌症模型和流行病学研究，IGFBP-3似乎可作为低外显率的肿瘤抑制基因。失调的IGFBP-3参与许多癌症。血清中IGFBP-3水平可用来筛选生长激素是否缺乏。在AIDS、不受控制的糖尿病、创伤和严重烧伤患者的血清中，IGFBP-3水平是下降的。

人 Human IGFBP3 分子靶点信息

分子名：IGFBP3, insulin like growth factor binding protein 3
别称：
- acid stable subunit of the 140 K IGF complex
- binding protein 29
- binding protein 53
- BP-53
- growth hormone-dependent binding protein
- IBP-3
- IBP3
- IGF-binding protein 3
- IGFBP-3
- insulin-like growth factor binding protein 3
- insulin-like growth factor-binding protein 3
基因序列：NCBI_Gene: 3486
蛋白序列：UniProtKB: P17936

人 Human IGFBP3靶点分子功能（预测）

Enables insulin-like growth factor I binding activity and protein tyrosine phosphatase activator activity. Involved in several processes, including negative regulation of smooth muscle cell migration; negative regulation of smooth muscle cell proliferation; and positive regulation of myoblast differentiation. Acts upstream of or within negative regulation of cell population proliferation; negative regulation of protein phosphorylation; and protein phosphorylation. Located in extracellular space and nucleus. Part of insulin-like growth factor ternary complex. Implicated in several diseases, including Alzheimer's disease; Turner syndrome; diabetes mellitus (multiple); prostate cancer; and transitional cell carcinoma. Biomarker of several diseases, including Barrett's esophagus; Down syndrome; autoimmune disease (multiple); bone disease (multiple); and urinary system cancer (multiple).