EK133HS

Human IL-33 High Sensitivity ELISA Kit检测试剂盒（酶联免疫吸附法）

¥2,000.00 – ¥3,400.00

因产品会迭代升级，具体实验步骤请按纸质版说明书操作

说明书

在售SKU:70-EK133HS-48, 70-EK133HS-96

种属：人
样本类型：血清、血浆、细胞培养上清及其他生物学样本
检测样本体积：血清血浆：20μL；细胞培养上清：100μL
灵敏度：0.16pg/mL
检测范围：6.25-400pg/mL
回收率：92%-110%

¥<\/span>2,000.00<\/bdi><\/span><\/span>","sku":"70-EK133HS-48","variation_description":"","variation_id":10312,"variation_is_active":true,"variation_is_visible":true,"weight":"","weight_html":"N\/A"},{"attributes":{"attribute_size":"96T"},"availability_html":"","backorders_allowed":false,"dimensions":{"length":"","width":"","height":""},"dimensions_html":"N\/A","display_price":3400,"display_regular_price":3400,"image":{"title":"ELISA-1.jpg","caption":"","url":"https:\/\/ecdn.liankebio.cn\/wp-content\/uploads\/ELISA-1.jpg","alt":"","src":"https:\/\/ecdn.liankebio.cn\/wp-content\/uploads\/ELISA-1.jpg","srcset":"https:\/\/www.liankebio.com\/wp-content\/uploads\/ELISA-1.jpg 800w, https:\/\/www.liankebio.com\/wp-content\/uploads\/ELISA-1-768x576.jpg 768w","sizes":"(max-width: 800px) 100vw, 800px","full_src":"https:\/\/ecdn.liankebio.cn\/wp-content\/uploads\/ELISA-1.jpg","full_src_w":800,"full_src_h":600,"gallery_thumbnail_src":"https:\/\/ecdn.liankebio.cn\/wp-content\/uploads\/ELISA-1-100x100.jpg","gallery_thumbnail_src_w":100,"gallery_thumbnail_src_h":100,"thumb_src":"https:\/\/ecdn.liankebio.cn\/wp-content\/uploads\/ELISA-1.jpg","thumb_src_w":800,"thumb_src_h":600,"src_w":800,"src_h":600},"image_id":5986,"is_downloadable":false,"is_in_stock":true,"is_purchasable":true,"is_sold_individually":"no","is_virtual":false,"max_qty":"","min_qty":1,"price_html":"¥<\/span>3,400.00<\/bdi><\/span><\/span>","sku":"70-EK133HS-96","variation_description":"","variation_id":10313,"variation_is_active":true,"variation_is_visible":true,"weight":"","weight_html":"N\/A"}]">

Size
库存号

ELISA试剂盒详细信息

商品名	Human IL-33 High Sensitivity ELISA Kit检测试剂盒（酶联免疫吸附法）
种属	人
检测方法	双抗体夹心法
检测样本类型	血清、血浆、细胞培养上清及其他生物学样本
检测样本体积	血清血浆：20μL；细胞培养上清：100μL
灵敏度	0.16pg/mL
线性范围	6.25-400pg/mL
精密度	板内变异系数：4.0%-4.6%;板间变异系数：2.6%-4.8%
回收率	92%-110%
平均回收率	99%
板式	96孔板，可拆
保存条件	2-8℃保存。已拆开：标准品-20℃保存，其它4℃。
运输条件	2-8℃冰袋运输
检测原理	本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人IL-33抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品和待测样本，经过孵育，样本中存在的IL-33与固相抗体结合。洗涤去除未结合的物质后，加入生物素化的检测抗体孵育。洗涤去除未结合的生物素化的抗体，加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后，加入信号增强剂孵育，洗涤去除未结合的物质后，再次加入Streptavidin-HRP。洗涤后，加入显色底物TMB，避光显色。颜色反应的深浅与样本中IL-33的浓度成正比。加入终止液终止反应，在450nm波长(参考波长570 - 630nm)测定吸光度值。