EK1282

Human Ki-67/MKI67 ELISA Kit检测试剂盒（酶联免疫吸附法）

¥1,600.00 – ¥2,650.00

因产品会迭代升级，具体实验步骤请按纸质版说明书操作

说明书

在售SKU:70-EK1282-48, 70-EK1282-96, EK1282

分子靶点：MKI67
种属：人
样本类型：血清、血浆、细胞培养上清及其他生物学样本
检测样本体积：50μL
灵敏度：0.35pg/mL
检测范围：6.25-400pg/mL
回收率：85%-102%

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Size
库存号

ELISA试剂盒详细信息

商品名	Human Ki-67/MKI67 ELISA Kit检测试剂盒（酶联免疫吸附法）
种属	人
靶点	MKI67
检测方法	双抗体夹心法
检测样本类型	血清、血浆、细胞培养上清及其他生物学样本
检测样本体积	50μL
灵敏度	0.35pg/mL
线性范围	6.25-400pg/mL
精密度	板内变异系数：1.9%-2.5%;板间变异系数：2.4%-3.0%
回收率	85%-102%
平均回收率	95%
板式	96孔板，可拆
保存条件	2-8℃保存。已拆开：标准品-20℃保存，其它4℃。
运输条件	2-8℃冰袋运输
检测原理	本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人Ki-67抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体，经过孵育，样本中存在的Ki-67与固相抗体和检测抗体结合。洗涤去除未结合的物质后，加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后，加入显色底物TMB，避光显色。颜色反应的深浅与样本中Ki-67的浓度成正比。加入终止液终止反应，在450nm波长(参考波长570 - 630nm)测定吸光度值。

文章目录[隐藏]

分子信息
MKI67 分子靶点信息概述
MKI67 分子靶点综述
- 人 Human MKI67 分子靶点信息
- 人 Human MKI67靶点分子功能（预测）

分子信息

概述人 MKI67, Ki-67, MIB-1 靶点信息

MKI67 分子靶点信息概述

分子名：MKI67, marker of proliferation Ki-67
基因家族：Protein phosphatase 1 regulatory subunits
别名：MIB-1; PPP1R105; Ki-67
全称：protein phosphatase 1, regulatory subunit 105; Molecular Immunology Borstel antibody 1; antigen identified by monoclonal antibody Ki-67

MKI67 分子靶点综述

KI-67也称为Ki-67或MKI67，在人类中是一种由MKI67基因编码的蛋白质。Ki-67是350 - 400kDa的核蛋白，属于染色体有丝分裂相关蛋白的成员。此外，Ki-67与核糖体RNA转录有关，其失活会抑制核糖体RNA的合成。Ki-67与160kDa 的Hklp2相互作用，后者促进中心体分离和纺锤体的两极性。它也直接与NIFK相互作用，并结合UBF，从而在核糖体RNA的合成中发挥作用。在ER阳性乳腺癌患者中，Ki-67代表了一群高增殖性的亚群，使他们在辅助化疗中获益更多。

人 Human MKI67 分子靶点信息

分子名：MKI67, marker of proliferation Ki-67
别称：
- antigen identified by monoclonal antibody Ki-67
- antigen KI-67
- antigen Ki67
- KIA
- MIB-
- MIB-1
- Molecular Immunology Borstel antibody 1
- PPP1R105
- proliferation marker protein Ki-67
- proliferation-related Ki-67 antigen
基因序列：NCBI_Gene: 4288
蛋白序列：UniProtKB: P46013

人 Human MKI67靶点分子功能（预测）

Enables protein C-terminus binding activity. Involved in regulation of chromosome segregation and regulation of mitotic nuclear division. Located in chromosome; nuclear body; and nucleolus. Colocalizes with condensed chromosome. Implicated in Crohn's disease; breast cancer; human immunodeficiency virus infectious disease; and pancreatic cancer. Biomarker of several diseases, including Barrett's esophagus; autoimmune disease of musculoskeletal system (multiple); endocrine gland cancer (multiple); gastrointestinal system cancer (multiple); and interstitial cystitis.