EK197

Human Leptin ELISA Kit检测试剂盒（酶联免疫吸附法）

¥1,600.00 – ¥2,650.00

因产品会迭代升级，具体实验步骤请按纸质版说明书操作

说明书

在售SKU:70-EK197-48,70-EK197-96, EK197, 人瘦素, 人, 瘦素

分子靶点：LEP
种属：人
样本类型：血清、血浆、细胞培养上清及其他生物学样本
检测样本体积：血清血浆：10μL；细胞培养上清：100μL
灵敏度：12.90pg/mL
检测范围：31.25-2000pg/mL
回收率：87%-112%

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Size
库存号

ELISA试剂盒详细信息

商品名	Human Leptin ELISA Kit检测试剂盒（酶联免疫吸附法）
种属	人
靶点	LEP
检测方法	双抗体夹心法
检测样本类型	血清、血浆、细胞培养上清及其他生物学样本
检测样本体积	血清血浆：10μL；细胞培养上清：100μL
灵敏度	12.90pg/mL
线性范围	31.25-2000pg/mL
精密度	板内变异系数：2.7%-4.4%;板间变异系数：2.5%-4.8%
回收率	87%-112%
平均回收率	98%
板式	96孔板，可拆
保存条件	2-8℃保存。已拆开：标准品-20℃保存，其它4℃。
运输条件	2-8℃冰袋运输
检测原理	本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人Leptin抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体，经过孵育，样本中存在的Leptin与固相抗体和检测抗体结合。洗涤去除未结合的物质后，加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后，加入显色底物TMB，避光显色。颜色反应的深浅与样本中Leptin的浓度成正比。加入终止液终止反应，在450nm波长(参考波长570 - 630nm)测定吸光度值。

文章目录[隐藏]

分子信息
LEP 分子靶点信息概述
LEP 分子靶点综述
- 人 Human LEP 分子靶点信息
- 人 Human LEP靶点分子功能（预测）

分子信息

概述人 LEP, OBS, OB 靶点信息

LEP 分子靶点信息概述

分子名：LEP, leptin
基因家族：Neuropeptides
曾用名：OBS; OB
全称：leptin (murine obesity homolog); leptin (obesity homolog, mouse)

LEP 分子靶点综述

瘦素是由脂肪细胞分泌的蛋白质类激素，可通过抑制饥饿来调控能量平衡。除了白色脂肪组织可以分泌瘦素外，褐色脂肪、胎盘、卵巢、骨骼肌、胃、乳腺上皮细胞、骨髓和P/D1细胞也能产生瘦素。瘦素和饥饿激素都作用于下丘脑弓状核的受体，以此来调节食欲，获得能量平衡。瘦素的基本功能是调节脂肪储存，但它在其它生理代谢过程中同样发挥作用，然而许多其它功能还未明确。研究表明瘦素与肥胖和减肥有关联。

人 Human LEP 分子靶点信息

分子名：LEP, leptin
别称：
- FLJ94114
- LEPD
- leptin (murine obesity homolog)
- leptin (obesity homolog, mouse)
- OB
- obese protein
- obese, mouse, homolog of
- obesity factor
- OBS
基因序列：NCBI_Gene: 3952
蛋白序列：UniProtKB: P41159

人 Human LEP靶点分子功能（预测）

Enables leptin receptor binding activity. Involved in several processes, including positive regulation of signal transduction; regulation of cytokine production; and regulation of lymphocyte proliferation. Predicted to be located in cytosol. Predicted to be active in extracellular space. Implicated in several diseases, including alcohol dependence; congenital leptin deficiency; liver disease (multiple); lung disease (multiple); and type 2 diabetes mellitus. Biomarker of several diseases, including alcohol dependence; cystic fibrosis; fatty liver disease (multiple); liver disease (multiple); and lung disease (multiple).