EK1M13

Human MMP-13 ELISA Kit检测试剂盒（酶联免疫吸附法）

¥1,600.00 – ¥2,650.00

因产品会迭代升级，具体实验步骤请按纸质版说明书操作

说明书

在售SKU: EK1M13, 70-EK1M13-48, 70-EK1M13-96

分子靶点：MMP13
种属：人
样本类型：血清、血浆、细胞培养上清及其他生物学样本
检测样本体积：血清、血浆样本50μL
灵敏度：0.43pg/mL
检测范围：31.25-2000pg/mL
回收率：89%-118%

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Size
库存号

ELISA试剂盒详细信息

商品名	Human MMP-13 ELISA Kit检测试剂盒（酶联免疫吸附法）
种属	人
靶点	MMP13
检测方法	双抗体夹心法
检测样本类型	血清、血浆、细胞培养上清及其他生物学样本
检测样本体积	血清、血浆样本50μL
灵敏度	0.43pg/mL
线性范围	31.25-2000pg/mL
精密度	板内变异系数：1.0%-1.9%;板间变异系数：4.5%-8.3%
回收率	89%-118%
平均回收率	100%
板式	96孔板，可拆
保存条件	2-8℃保存。已拆开：标准品-20℃保存，其它4℃。
运输条件	2-8℃冰袋运输
检测原理	本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人MMP-13抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体，经过孵育，样本中存在的MMP-13与固相抗体和检测抗体结合。洗涤去除未结合的物质后，加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后，加入显色底物TMB，避光显色。颜色反应的深浅与样本中MMP-13的浓度成正比。加入终止液终止反应，在450nm波长(参考波长570 - 630nm)测定吸光度值。

文章目录[隐藏]

分子信息
MMP13 分子靶点信息概述
MMP13 分子靶点综述
- 人 Human MMP13 分子靶点信息
- 人 Human MMP13靶点分子功能（预测）

分子信息

概述人 MMP13, MMP-13, CLG3 靶点信息

MMP13 分子靶点信息概述

分子名：MMP13, matrix metallopeptidase 13
基因家族：Receptor ligands; M10 matrix metallopeptidases
别名：CLG3
全称：collagenase 3; matrix metalloproteinase 13 (collagenase 3)

MMP13 分子靶点综述

基质金属蛋白酶(MMPs)是钙依赖性的含锌蛋白酶，其中有 26 个成员(MMP-1 至 MMP-26)已被确定。MMPs 参与正常生理过程(如胚胎发育、繁殖、组织重塑)和病理过程(如关节炎和转移)中细胞外基质的降解。MMP-13，又名胶原酶 3，在人类中由 MMP13 基因编码。与大多数 MMPs 一样，它以非活性的前体形式被分泌存在。前肽结构域一旦被裂解，MMP-13 就被激活，该活化酶由催化结构域和类血红素结构域 PDB: 1PEX 组成。在胚胎发育过程中，MMP-13 在骨骼中表达，对于骨矿化中胶原基质的重组是必不可少的。在病理情况下，如肿瘤、类风湿关节炎和骨关节炎，MMP-13 是高度过表达的。

人 Human MMP13 分子靶点信息

分子名：MMP13, matrix metallopeptidase 13
别称：
- CLG3
- collagenase 3
- MANDP1
- matrix metallopeptidase 13 (collagenase 3)
- matrix metalloproteinase 13 (collagenase 3)
- MDST
- MMP-13
基因序列：NCBI_Gene: 4322
蛋白序列：UniProtKB: P45452

人 Human MMP13靶点分子功能（预测）

Enables collagen binding activity; metal ion binding activity; and metalloendopeptidase activity. Involved in bone morphogenesis; collagen catabolic process; and extracellular matrix disassembly. Predicted to be located in extracellular space. Predicted to be part of collagen trimer. Implicated in anodontia; artery disease (multiple); osteoarthritis; ovarian carcinoma; and spondyloepimetaphyseal dysplasia, Missouri type. Biomarker of invasive ductal carcinoma; reproductive organ cancer (multiple); and urinary bladder cancer.