EK1M02

Human MMP-2 ELISA Kit检测试剂盒（酶联免疫吸附法）

¥1,600.00 – ¥2,650.00

因产品会迭代升级，具体实验步骤请按纸质版说明书操作

说明书

在售SKU:70-EK1M02-48, 70-EK1M02-96, EK1M02

分子靶点：MMP2
种属：人
样本类型：血清、血浆、细胞培养上清及其他生物学样本
检测样本体积：血清、血浆样本10μL
灵敏度：69.14pg/mL
检测范围：2-128ng/mL
回收率：86%-118%

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Size
库存号

ELISA试剂盒详细信息

商品名	Human MMP-2 ELISA Kit检测试剂盒（酶联免疫吸附法）
种属	人
靶点	MMP2
检测方法	双抗体夹心法
检测样本类型	血清、血浆、细胞培养上清及其他生物学样本
检测样本体积	血清、血浆样本10μL
灵敏度	69.14pg/mL
线性范围	2-128ng/mL
精密度	板内变异系数：2.1%-7.8%;板间变异系数：2.4%-3.8%
回收率	86%-118%
平均回收率	108%
板式	96孔板，可拆
保存条件	2-8℃保存。已拆开：标准品-20℃保存，其它4℃。
运输条件	2-8℃冰袋运输
检测原理	本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人MMP-2抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体，经过孵育，样本中存在的MMP-2与固相抗体和检测抗体结合。洗涤去除未结合的物质后，加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后，加入显色底物TMB，避光显色。颜色反应的深浅与样本中MMP-2的浓度成正比。加入终止液终止反应，在450nm波长(参考波长570 - 630nm)测定吸光度值。

文章目录[隐藏]

分子信息
MMP2 分子靶点信息概述
MMP2 分子靶点综述
- 人 Human MMP2 分子靶点信息
- 人 Human MMP2靶点分子功能（预测）

分子信息

概述人 MMP2, MMP-2, TBE-1 靶点信息

MMP2 分子靶点信息概述

分子名：MMP2, matrix metallopeptidase 2
基因家族：M10 matrix metallopeptidases
别名：TBE-1
曾用名：CLG4; CLG4A
全称：matrix metalloproteinase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase)

MMP2 分子靶点综述

基质金属蛋白酶2(MMP-2)，又名72kDa的IV型胶原酶和明胶酶A，在人类中由MMP2基因编码。激活MMP-2需要蛋白水解加工。MMP-2在子宫内膜破裂、调节血管形成和炎症应答中起作用。MMP-2参与许多事件，如癌症进程、癌细胞浸润、细胞信号转导、新血管形成和淋巴管生成。MMP2基因突变与Torg-Winchester综合征、多发性骨溶解、关节炎综合征有关，可能与瘢痕疙瘩有关。非囊性纤维化支气管扩张中，相比于呼吸道感染铜绿假单胞菌的患者，感染流感嗜血杆菌患者的MMP-2浓度是升高的。

人 Human MMP2 分子靶点信息

分子名：MMP2, matrix metallopeptidase 2
别称：
- 72 kDa gelatinase
- 72 kDa type IV collagenase
- CLG4
- CLG4A
- collagenase type IV-A
- gelatinase A
- matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase)
- matrix metalloproteinase 2 (gelatinase a, 72kda gelatinase, 72kda type iv collagenase)
- matrix metalloproteinase-2
- matrix metalloproteinase-II
- MMP-2
- MMP-II
- MONA
- neutrophil gelatinase
- TBE-1
基因序列：NCBI_Gene: 4313
蛋白序列：UniProtKB: P08253

人 Human MMP2靶点分子功能（预测）

Enables metallopeptidase activity and serine-type endopeptidase activity. Involved in several processes, including cellular response to UV-A; endodermal cell differentiation; and positive regulation of vascular associated smooth muscle cell proliferation. Located in extracellular space. Colocalizes with collagen-containing extracellular matrix. Implicated in artery disease (multiple); eye disease (multiple); pseudoxanthoma elasticum; and temporal arteritis. Biomarker of several diseases, including artery disease (multiple); carcinoma (multiple); cardiomyopathy (multiple); cholesteatoma (multiple); and neurodegenerative disease (multiple).