EK1393

Human NT-proBNP ELISA Kit 检测试剂盒（酶联免疫吸附法）

¥1,600.00 – ¥2,650.00

因产品会迭代升级，具体实验步骤请按纸质版说明书操作

说明书

在售SKU:70-EK1393-48, 70-EK1393-96, EK1393

分子靶点：NPPB
种属：人
样本类型：血清、血浆、细胞培养上清及其他生物学样本
检测样本体积：血清样本20μL
灵敏度：4.56pg/mL
检测范围：31.25-2000pg/mL
回收率：91%-119%

¥<\/span>1,600.00<\/bdi><\/span><\/span>","sku":"70-EK1393-48","variation_description":"","variation_id":18430,"variation_is_active":true,"variation_is_visible":true,"weight":"","weight_html":"N\/A"},{"attributes":{"attribute_pa_size":"96-tests"},"availability_html":"","backorders_allowed":false,"dimensions":{"length":"","width":"","height":""},"dimensions_html":"N\/A","display_price":2650,"display_regular_price":2650,"image":{"title":"ELISA-1.jpg","caption":"","url":"https:\/\/ecdn.liankebio.cn\/wp-content\/uploads\/ELISA-1.jpg","alt":"","src":"https:\/\/ecdn.liankebio.cn\/wp-content\/uploads\/ELISA-1.jpg","srcset":"https:\/\/www.liankebio.com\/wp-content\/uploads\/ELISA-1.jpg 800w, https:\/\/www.liankebio.com\/wp-content\/uploads\/ELISA-1-768x576.jpg 768w","sizes":"(max-width: 800px) 100vw, 800px","full_src":"https:\/\/ecdn.liankebio.cn\/wp-content\/uploads\/ELISA-1.jpg","full_src_w":800,"full_src_h":600,"gallery_thumbnail_src":"https:\/\/ecdn.liankebio.cn\/wp-content\/uploads\/ELISA-1-100x100.jpg","gallery_thumbnail_src_w":100,"gallery_thumbnail_src_h":100,"thumb_src":"https:\/\/ecdn.liankebio.cn\/wp-content\/uploads\/ELISA-1.jpg","thumb_src_w":800,"thumb_src_h":600,"src_w":800,"src_h":600},"image_id":5986,"is_downloadable":false,"is_in_stock":true,"is_purchasable":true,"is_sold_individually":"no","is_virtual":false,"max_qty":"","min_qty":1,"price_html":"¥<\/span>2,650.00<\/bdi><\/span><\/span>","sku":"70-EK1393-96","variation_description":"","variation_id":18431,"variation_is_active":true,"variation_is_visible":true,"weight":"","weight_html":"N\/A"}]">

Size
库存号

ELISA试剂盒详细信息

商品名	Human NT-proBNP ELISA Kit 检测试剂盒（酶联免疫吸附法）
种属	人
靶点	NPPB
检测方法	双抗体夹心法
检测样本类型	血清、血浆、细胞培养上清及其他生物学样本
检测样本体积	血清样本20μL
灵敏度	4.56pg/mL
线性范围	31.25-2000pg/mL
精密度	板内变异系数：4.1%-6.0%;板间变异系数：3.7%-4.1%
回收率	91%-119%
平均回收率	106%
板式	96孔板，可拆
保存条件	2-8℃保存。已拆开：标准品-20℃保存，其它4℃。
运输条件	2-8℃冰袋运输
检测原理	本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性捕获抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体，经过孵育，样本中存在的待测物质与捕获抗体和检测抗体结合。洗涤去除未结合的物质后，加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后，加入显色底物（TMB），避光显色。颜色反应的深浅与样本中待测物质的浓度成正比。加入终止液终止反应，在450nm波长(参考波长570 - 630nm)测定吸光度值。

文章目录[隐藏]

NPPB 分子靶点信息概述
NPPB 分子靶点综述
NPPB, natriuretic peptide B 分子靶点种属信息
- 人 Human NPPB 分子靶点信息
- 人 Human NPPB靶点分子功能（预测）

NPPB 分子靶点信息概述

分子名：NPPB, natriuretic peptide B
基因家族：Neuropeptides
曾用名：natriuretic peptide precursor B
全称：natriuretic peptide precursor B

NPPB 分子靶点综述

脑型利钠肽(Brain-type Natriuretic Peptide, BNP)是一种非糖基化肽，主要由心室肌细胞产生，属于利钠肽家族。12 kDa BNP 前体蛋白水解裂解产生 N 端 proBNP (NT-proBNP)和成熟的 BNP。血浆 NT-proBNP 是充血性心力衰竭的标志，而成熟 BNP (AA 103 - 134)促进血管扩张和液体和钠的排泄。人类 BNP 前体与小鼠和猪 BNP 前体的 AA 序列同源性分别为29%和 51%。 BNP 是一种心脏激素，可能作为心脏旁分泌抗纤维化因子。通过利钠、利尿、血管舒张和抑制肾素和醛固酮分泌，也在心血管内稳态中发挥关键作用。特异性结合和刺激 NPR1 受体的 cGMP 产生。与清除受体 NPR3 结合。应用于肿瘤，心血管疾病等。

NPPB, natriuretic peptide B 分子靶点种属信息

人 Human

人 Human NPPB 分子靶点信息

分子名：NPPB, natriuretic peptide B
别称：
- BNP
- brain natriuretic factor prohormone
- brain type natriuretic peptide
- gamma-brain natriuretic peptide
- Iso-ANP
- natriuretic peptide precursor B
- natriuretic peptides B
- natriuretic protein
- preproBNP
- proBNP
基因序列：NCBI_Gene: 4879
蛋白序列：UniProtKB: P16860

人 Human NPPB靶点分子功能（预测）

Enables hormone receptor binding activity. Involved in several processes, including antimicrobial humoral immune response mediated by antimicrobial peptide; killing of cells of another organism; and receptor guanylyl cyclase signaling pathway. Part of protein-containing complex. Implicated in atherosclerosis; hypertension; myocardial infarction; obesity; and systolic heart failure. Biomarker of several diseases, including Kawasaki disease; artery disease (multiple); diabetes mellitus (multiple); heart disease (multiple); and kidney failure (multiple).