EK1135

Human osteopontin/OPN ELISA Kit检测试剂盒（酶联免疫吸附法）

¥1,600.00 – ¥2,650.00

因产品会迭代升级，具体实验步骤请按纸质版说明书操作

说明书

在售SKU:70-EK1135-48, 70-EK1135-96, EK1135

分子靶点：SPP1
种属：人
样本类型：血清、血浆、细胞培养上清及其他生物学样本
检测样本体积：血清、血浆样本5μL
灵敏度：4.42pg/mL
检测范围：125-8000pg/mL
回收率：75%-126%

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Size
库存号

ELISA试剂盒详细信息

商品名	Human osteopontin/OPN ELISA Kit检测试剂盒（酶联免疫吸附法）
种属	人
靶点	SPP1
检测方法	双抗体夹心法
检测样本类型	血清、血浆、细胞培养上清及其他生物学样本
检测样本体积	血清、血浆样本5μL
灵敏度	4.42pg/mL
线性范围	125-8000pg/mL
精密度	板内变异系数：4.1%-5.3%;板间变异系数：7.2%-9%
回收率	75%-126%
平均回收率	99%
板式	96孔板，可拆
保存条件	2-8℃保存。已拆开：标准品-20℃保存，其它4℃。
运输条件	2-8℃冰袋运输
检测原理	本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人OPN抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品和待测样本，经过孵育，样本中存在的OPN与固相抗体结合。洗涤去除未结合的物质后，加入生物素化的检测抗体孵育。洗涤去除未结合的生物素化的抗体，加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后，加入显色底物TMB，避光显色。颜色反应的深浅与样本中OPN的浓度成正比。加入终止液终止反应，在450nm波长(参考波长570 - 630nm)测定吸光度值。

文章目录[隐藏]

分子信息
SPP1 分子靶点信息概述
SPP1 分子靶点综述
- 人 Human SPP1 分子靶点信息
- 人 Human SPP1靶点分子功能（预测）

分子信息

概述人 SPP1, OPN, Osteopontin 靶点信息

SPP1 分子靶点信息概述

分子名：SPP1, secreted phosphoprotein 1
基因家族：Receptor ligands; SIBLING family
别名：BSPI; ETA-1
曾用名：BNSP; OPN
全称：early T-lymphocyte activation 1; osteopontin; bone sialoprotein I

SPP1 分子靶点综述

骨桥蛋白(OPN)，又名BSP-1、ETA-1、SPP-1或Ric，在人类中由SPP1基因编码，主要在骨骼中表达。OPN基因的调节机制还未完全阐明，不同细胞类型的调节机理是不同的。OPN在生物矿化、骨重塑、免疫功能、趋化、细胞活化和凋亡上有作用，同样与心肌细胞凋亡和心肌功能紊乱的增加有关。OPN与许多生理和病理过程包括伤口愈合、骨转换、肿瘤发生、炎症、缺血和免疫应答有关。调控血浆(或局部)的OPN水平对治疗自身免疫性疾病、癌症转移、骨和牙齿的矿化、骨质疏松症和一些应激形式有帮助。

人 Human SPP1 分子靶点信息

分子名：SPP1, secreted phosphoprotein 1
别称：
- BNSP
- bone sialoprotein 1
- bone sialoprotein I
- BSPI
- early T-lymphocyte activation 1
- ETA-1
- MGC110940
- nephropontin
- OPN
- osteopontin
- osteopontin-C
- osteopontin-D
- osteopontin/immunoglobulin alpha 1 heavy chain constant region fusion protein
- secreted phosphoprotein 1 (osteopontin, bone sialoprotein I, early T-lymphocyte activation 1)
- secreted phosphoprotein 1 variant 6
- secreted phosphoprotein-1 (osteopontin, bone sialoprotein)
- SPP-1
- SPP1/CALPHA1 fusion
- urinary stone protein
- uropontin
基因序列：NCBI_Gene: 6696
蛋白序列：UniProtKB: P10451

人 Human SPP1靶点分子功能（预测）

Enables integrin binding activity. Involved in several processes, including androgen catabolic process; cellular response to testosterone stimulus; and positive regulation of estradiol secretion. Located in Golgi apparatus and extracellular space. Implicated in several diseases, including autoimmune disease (multiple); biliary atresia; coronary artery disease (multiple); disease of cellular proliferation (multiple); and hepatitis. Biomarker of calcinosis; coronary artery disease; kidney failure; progressive osseous heteroplasia; and urinary bladder cancer.