EK188

Human sCD40L ELISA Kit检测试剂盒（酶联免疫吸附法）

¥1,600.00 – ¥2,650.00

因产品会迭代升级，具体实验步骤请按纸质版说明书操作

说明书

在售SKU:70-EK188-48, 70-EK188-96, EK188

分子靶点：CD40LG
种属：人
样本类型：血清、血浆、细胞培养上清及其他生物学样本
检测样本体积：血清、血浆样本20μL
灵敏度：4.09pg/mL
检测范围：62.5-4000pg/mL
回收率：88%-110%

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Size
库存号

ELISA试剂盒详细信息

商品名	Human sCD40L ELISA Kit检测试剂盒（酶联免疫吸附法）
种属	人
靶点	CD40LG
检测方法	双抗体夹心法
检测样本类型	血清、血浆、细胞培养上清及其他生物学样本
检测样本体积	血清、血浆样本20μL
灵敏度	4.09pg/mL
线性范围	62.5-4000pg/mL
精密度	板内变异系数：5.2%-6.2%;板间变异系数：3.2%-8.4%
回收率	88%-110%
平均回收率	101%
板式	96孔板，可拆
保存条件	2-8℃保存。已拆开：标准品-20℃保存，其它4℃。
运输条件	2-8℃冰袋运输
检测原理	本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人sCD40L抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体，经过孵育，样本中存在的sCD40L与固相抗体和检测抗体结合。洗涤去除未结合的物质后，加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后，加入显色底物TMB，避光显色。颜色反应的深浅与样本中sCD40L的浓度成正比。加入终止液终止反应，在450nm波长(参考波长570 - 630nm)测定吸光度值。

文章目录[隐藏]

分子信息
CD40LG 分子靶点信息概述
CD40LG 分子靶点综述
- 人 Human CD40LG 分子靶点信息
- 人 Human CD40LG靶点分子功能（预测）

分子信息

概述人 CD40LG, CD40L, TNFSF5, CD154 靶点信息

CD40LG 分子靶点信息概述

分子名：CD40LG, CD40 ligand
基因家族：CD molecules; Tumor necrosis factor superfamily
别名：CD40L; TRAP; gp39; hCD40L; CD154
曾用名：HIGM1; IMD3; TNFSF5
全称：CD40 antigen ligand; tumor necrosis factor (ligand) superfamily member 5; T-B cell-activating molecule; TNF-related activation protein; hyper-IgM syndrome; tumor necrosis factor (ligand) superfamily, member 5 (hyper-IgM syndrome)

CD40LG 分子靶点综述

CD40配体(CD40L/CD154)是TNF分子超家族中的一员，它是一种分子量为39kDa的糖蛋白，主要表达于活化的T细胞。分子量为15-18kDa的可溶性CD40L(sCD40L)通过蛋白水解产生。CD40L与抗原提呈细胞(APC)表面的CD40结合，根据不同的靶细胞类型可产生多种效果。CD40L作为共刺激分子，对T细胞亚群滤泡辅助性T细胞(Tfh细胞)尤其重要。在Tfh细胞中，CD40L与B细胞表面的CD40结合，促进B细胞成熟和功能发挥，从而促进细胞与细胞之间的联系。CD40L基因的缺失导致无法发生免疫球蛋白类型转换，且与高IgM综合征有关。CD40L缺失也会阻断生发中心形成，因此阻止适应性免疫系统中的重要过程即抗体亲和力成熟。

人 Human CD40LG 分子靶点信息

分子名：CD40LG, CD40 ligand
别称：
- CD154
- CD40 antigen ligand
- CD40-L
- CD40L
- gp39
- hCD40L
- HIGM1
- IGM
- IMD3
- T-B cell-activating molecule
- T-BAM
- T-cell antigen Gp39
- TNF-related activation protein
- TNFSF5
- TRAP
- tumor necrosis factor (ligand) superfamily member 5
基因序列：NCBI_Gene: 959
蛋白序列：UniProtKB: P29965

人 Human CD40LG靶点分子功能（预测）

Enables CD40 receptor binding activity and integrin binding activity. Involved in several processes, including cell surface receptor signaling pathway; positive regulation of cytokine production; and regulation of apoptotic process. Located in cell surface. Implicated in several diseases, including Kawasaki disease; autoimmune disease (multiple); combined T cell and B cell immunodeficiency (multiple); hematologic cancer (multiple); and lupus nephritis. Biomarker of several diseases, including allergic rhinitis; autoimmune disease (multiple); cardiovascular system disease (multiple); diabetes mellitus (multiple); and hematologic cancer (multiple).