EK190

Human sVCAM-1/CD106 ELISA Kit检测试剂盒（酶联免疫吸附法）

¥1,600.00 – ¥2,650.00

因产品会迭代升级，具体实验步骤请按纸质版说明书操作

说明书

在售SKU:70-EK190-48, 70-EK190-96， EK190

分子靶点：VCAM1
种属：人
样本类型：血清、血浆、细胞培养上清及其他生物学样本
检测样本体积：血清、血浆样本10μL
灵敏度：2.65pg/mL
检测范围：31.25-2000pg/mL
回收率：86%-111%

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Size
库存号

ELISA试剂盒详细信息

商品名	Human sVCAM-1/CD106 ELISA Kit检测试剂盒（酶联免疫吸附法）
种属	人
靶点	VCAM1
检测方法	双抗体夹心法
检测样本类型	血清、血浆、细胞培养上清及其他生物学样本
检测样本体积	血清、血浆样本10μL
灵敏度	2.65pg/mL
线性范围	31.25-2000pg/mL
精密度	板内变异系数：1.9%-2.6%;板间变异系数：3.2%-5.0%
回收率	86%-111%
平均回收率	99%
板式	96孔板，可拆
保存条件	2-8℃保存。已拆开：标准品-20℃保存，其它4℃。
运输条件	2-8℃冰袋运输
检测原理	本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人sVCAM-1抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体，经过孵育，样本中存在的sVCAM-1与固相抗体和检测抗体结合。洗涤去除未结合的物质后，加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后，加入显色底物TMB，避光显色。颜色反应的深浅与样本中sVCAM-1的浓度成正比。加入终止液终止反应，在450nm波长(参考波长570 - 630nm)测定吸光度值。

文章目录[隐藏]

分子信息
VCAM1 分子靶点信息概述
VCAM1 分子靶点综述
- 人 Human VCAM1 分子靶点信息
- 人 Human VCAM1靶点分子功能（预测）

分子信息

概述人 VCAM1, CD106 靶点信息

VCAM1 分子靶点信息概述

分子名：VCAM1, vascular cell adhesion molecule 1
基因家族：CD molecules; Receptor ligands; C2-set domain containing; I-set domain containing; Ig-like cell adhesion molecule family
别名：CD106

VCAM1 分子靶点综述

血管细胞粘附分子1(VCAM-1)也称为CD106，是100-110kDaI型跨膜蛋白，通常具有7个C2型免疫球蛋白结构域，作为细胞黏附分子发挥功能。VCAM-1蛋白介导淋巴细胞、单核细胞、嗜酸性粒细胞和嗜碱性粒细胞等黏附于血管内皮。VCAM-1是整合素β1亚家族VLA-4(极迟抗原-4或α4β1)和整合素α4β7的内皮配体。在其他类型细胞上也观察到VCAM-1的表达。它也能与EZR和膜突蛋白作用。VCAM-1的其它功能包括通过细胞-细胞接触调控破骨细胞生成、血氧不足时诱导镰状细胞黏附于血管内皮细胞。它还在白细胞-内皮细胞信号传导中发挥作用。监测血清中的sVCAM-1表达水平可为多种病理状态提供更详细的视角，如动脉粥样硬化和类风湿性关节炎。

人 Human VCAM1 分子靶点信息

分子名：VCAM1, vascular cell adhesion molecule 1
别称：
- CD106
- CD106 antigen
- DKFZp779G2333
- INCAM-100
- MGC99561
- vascular cell adhesion protein 1
基因序列：NCBI_Gene: 7412
蛋白序列：UniProtKB: P19320

人 Human VCAM1靶点分子功能（预测）

Enables integrin binding activity and primary amine oxidase activity. Involved in several processes, including calcium-mediated signaling using intracellular calcium source; cell-cell adhesion in response to extracellular stimulus; and cellular response to amyloid-beta. Located in several cellular components, including early endosome; external side of plasma membrane; and podosome. Part of alpha9-beta1 integrin-vascular cell adhesion molecule-1 complex. Biomarker of several diseases, including artery disease (multiple); diabetes mellitus (multiple); essential thrombocythemia; glomerulonephritis (multiple); and interstitial cystitis.