EK295

Mouse Adiponectin ELISA Kit检测试剂盒（酶联免疫吸附法）

¥1,600.00 – ¥2,650.00

因产品会迭代升级，具体实验步骤请按纸质版说明书操作

说明书

在售SKU:70-EK295/2-48, 70-EK295/2-96, EK295

分子靶点：ADIPOQ
种属：小鼠
样本类型：血清、血浆、细胞培养上清及其他生物学样本
检测样本体积：血清、血浆样本10μL
灵敏度：0.73pg/mL
检测范围：31.25-2000pg/mL
回收率：92%-113%

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Size
库存号

ELISA试剂盒详细信息

商品名	Mouse Adiponectin ELISA Kit检测试剂盒（酶联免疫吸附法）
种属	小鼠
靶点	ADIPOQ
检测方法	双抗体夹心法
检测样本类型	血清、血浆、细胞培养上清及其他生物学样本
检测样本体积	血清、血浆样本10μL
灵敏度	0.73pg/mL
线性范围	31.25-2000pg/mL
精密度	板内变异系数：5.1%-8.8%;板间变异系数：7.3%-7.7%
回收率	92%-113%
平均回收率	107%
板式	96孔板，可拆
保存条件	2-8℃保存。已拆开：标准品-20℃保存，其它4℃。
运输条件	2-8℃冰袋运输
检测原理	本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗小鼠Adiponectin抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体，经过孵育，样本中存在的Adiponectin与固相抗体和检测抗体结合。洗涤去除未结合的物质后，加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后，加入显色底物TMB，避光显色。颜色反应的深浅与样本中Adiponectin的浓度成正比。加入终止液终止反应，在450nm波长(参考波长570 - 630nm)测定吸光度值。

文章目录[隐藏]

分子信息
ADIPOQ 分子靶点信息概述
ADIPOQ 分子靶点综述
- 小鼠 Mouse Adipoq 分子靶点信息
- 小鼠 Mouse Adipoq 靶点分子功能（预测）

分子信息

概述小鼠 ADIPOQ, ACRP30, GBP28 靶点信息

ADIPOQ 分子靶点信息概述

分子名：ADIPOQ, adiponectin, C1Q and collagen domain containing
基因家族：C1q and TNF related
别名：ACRP30; apM1; GBP28; adiponectin
曾用名：ACDC
全称：adipose most abundant gene transcript 1; adiponectin precursor; adipocyte, C1Q and collagen domain containing

ADIPOQ 分子靶点综述

脂联素，又称脂肪细胞补体相关蛋白30(Acrp30)，参与调节葡萄糖水平和脂肪酸分解。它组装成不同的复合物包括三聚体、六聚体和高阶寡聚体结构，进而可能影响生物活性。脂联素是一种蛋白质激素，调节许多代谢过程，包括葡萄糖调节和脂肪酸氧化。脂肪组织(以及妊娠期的胎盘)专门分泌脂联素到血液中，因此相对于其它许多激素，血浆中的脂联素含量很丰富。脂联素可能具有抗动脉粥样硬化和消炎的作用，可负向调节粒单祖细胞的生长和TNF-α在巨噬细胞中的产生。监测一些病理状态下的血清脂联素水平，如2型糖尿病、肥胖、动脉粥样硬化和非酒精性脂肪肝疾病，可为我们了解脂联素提供更详细的见解。

小鼠 Mouse Adipoq 分子靶点信息

分子名：Adipoq, adiponectin, C1Q and collagen domain containing
别称：
- Acdc
- Acrp30
- Ad
- adipo
- adipocyte complement related protein
- adipocyte, C1Q and collagen domain containing
- adiponectin
- apM1
- APN
- GBP28
基因序列：NCBI_Gene: 11450
蛋白序列：

小鼠 Mouse Adipoq 靶点分子功能（预测）

Enables hormone activity and identical protein binding activity. Involved in several processes, including detection of oxidative stress; negative regulation of cold-induced thermogenesis; and regulation of protein serine/threonine kinase activity. Acts upstream of or within with a positive effect on gene expression. Acts upstream of or within several processes, including brown fat cell differentiation; negative regulation of gluconeogenesis; and positive regulation of glucose import. Located in endoplasmic reticulum and extracellular space. Is expressed in several structures, including adipose tissue; axial musculature; gut; male reproductive gland or organ; and serum. Human ortholog(s) of this gene implicated in several diseases, including carcinoma (multiple); cardiovascular system disease (multiple); non-alcoholic fatty liver disease; primary open angle glaucoma; and type 2 diabetes mellitus. Orthologous to human ADIPOQ (adiponectin, C1Q and collagen domain containing).