EK201B

Mouse IL-1β ELISA Kit 检测试剂盒（酶联免疫吸附法）

Name: Mouse IL-1β ELISA Kit 检测试剂盒（酶联免疫吸附法）
SKU: EK201B
Availability: InStock

¥1,600.00 – ¥10,800.00

因产品会迭代升级，具体实验步骤请按纸质版说明书操作

在售SKU: EK201B, 70-EK201B/4-48, 70-EK201B/4-96, 70-EK201B/4-480

分子靶点：IL1B
种属：小鼠
样本类型：测血清、血浆和细胞培养上清
检测样本体积：血清血浆样本10μL
灵敏度：5.699pg/mL
检测范围：23.44-1500pg/mL
回收率：83%-96%

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Size
库存号

ELISA试剂盒详细信息

商品名	Mouse IL-1β ELISA Kit 检测试剂盒（酶联免疫吸附法）
种属	小鼠
靶点	IL1B
检测方法	双抗体夹心法
检测样本类型	测血清、血浆和细胞培养上清
检测样本体积	血清血浆样本10μL
灵敏度	5.699pg/mL
线性范围	23.44-1500pg/mL
精密度	板内变异系数：6.20%-7.19%;板间变异系数：3.90%-8.04%
回收率	83%-96%
平均回收率	90%
板式	96孔板，可拆
保存条件	2-8℃保存。已拆开：标准品-20℃保存，其它4℃。
运输条件	2-8℃冰袋运输
检测原理	本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性捕获抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体，经过孵育，样本中存在的待测物质与捕获抗体和检测抗体结合。洗涤去除未结合的物质后，加入辣根过氧化物酶标记的链霉亲和素 (Streptavidin-HRP)。洗涤后，加入显色底物（TMB），避光显色。颜色反应的深浅与样本中待测物质的浓度成正比。加入终止液终止反应，在450nm波长 (参考波长570 - 630nm) 测定吸光度值。

文章目录[隐藏]

分子信息
IL1B 分子靶点信息概述
IL1B 分子靶点综述
- 小鼠 Mouse Il1b 分子靶点信息
- 小鼠 Mouse Il1b 靶点分子功能（预测）

分子信息

概述小鼠 IL1B, IL1-β, IL1F2 靶点信息

IL1B 分子靶点信息概述

分子名：IL1B, interleukin 1 beta
基因家族：Interleukins
别名：IL1F2; IL-1B; IL1-BETA

IL1B 分子靶点综述

白细胞介素1(IL-1)是17kDa的胞外多肽，分为IL-1α和IL-1β两种蛋白。IL-1β是活化的巨噬细胞分泌的蛋白前体，通过caspase1(CASP1/ICE)水解产生的活化形式。该细胞因子是炎症反应的重要介导者，参与许多细胞活性，包括细胞增殖、分化和凋亡。IL-1是一类重要的蛋白家族，它们由生物活化的单核细胞所衍生，参与炎症反应和免疫应答。
IL-1β表达升高会产生各种不同的自身炎症综合征，最显著的是单基因条件称为CAPS，是由于触发IL-1β加工的炎性体受体NLRP3发生突变而引起的。

小鼠 Mouse Il1b 分子靶点信息

分子名：Il1b, interleukin 1 beta
别称：
- IL-1B
- Il-1b
- IL-1beta
基因序列：NCBI_Gene: 16176
蛋白序列：
- UniprotKB: P10749
- UniprotKB: Q3TI17

小鼠 Mouse Il1b 靶点分子功能（预测）

Enables cytokine activity. Involved in several processes, including negative regulation of cell communication; regulation of defense response; and regulation of lipid metabolic process. Acts upstream of or within several processes, including ectopic germ cell programmed cell death; positive regulation of intracellular signal transduction; and positive regulation of macromolecule metabolic process. Located in extracellular space and secretory granule. Is expressed in several structures, including dorsal aorta; early conceptus; hemolymphoid system; integument; and reproductive system. Human ortholog(s) of this gene implicated in several diseases, including autoimmune disease (multiple); blood platelet disease (multiple); eye disease (multiple); gastrointestinal system cancer (multiple); and lung disease (multiple). Orthologous to human IL1B (interleukin 1 beta).