EK282

Mouse TNF-a ELISA Kit检测试剂盒（酶联免疫吸附法）

¥1,600.00 – ¥10,800.00

因产品会迭代升级，具体实验步骤请按纸质版说明书操作

说明书

在售SKU:70-EK282-48, 70-EK282-96, EK282

分子靶点：TNF
种属：小鼠
样本类型：血清、血浆、细胞培养上清及其他生物学样本
检测样本体积：血清样本20μL
灵敏度：0.99pg/mL
检测范围：10.94-700pg/mL
回收率：81%-119%

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Size
库存号

ELISA试剂盒详细信息

商品名	Mouse TNF-a ELISA Kit检测试剂盒（酶联免疫吸附法）
种属	小鼠
靶点	TNF
检测方法	双抗体夹心法
检测样本类型	血清、血浆、细胞培养上清及其他生物学样本
检测样本体积	血清样本20μL
灵敏度	0.99pg/mL
线性范围	10.94-700pg/mL
精密度	板内变异系数：3.1%-4.5%;板间变异系数：3.8%-5.1%
回收率	81%-119%
平均回收率	101%
板式	96孔板，可拆
保存条件	2-8℃保存。已拆开：标准品-20℃保存，其它4℃。
运输条件	2-8℃冰袋运输
检测原理	本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性捕获抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本，经过孵育，样本中存在的待测物质与捕获抗体结合。洗涤去除未结合的物质后，加入辣根过氧化物酶标记的检测抗体孵育。洗涤后，加入显色底物（TMB），避光显色。颜色反应的深浅与样本中待测物质的浓度成正比。加入终止液终止反应，在450nm波长(参考波长570 - 630nm)测定吸光度值。

文章目录[隐藏]

分子信息
TNF 分子靶点信息概述
TNF 分子靶点综述
- 小鼠 Mouse Tnf 分子靶点信息
- 小鼠 Mouse Tnf 靶点分子功能（预测）

分子信息

概述小鼠 TNF, TNFA, TNFSF2 靶点信息

TNF 分子靶点信息概述

分子名：TNF, tumor necrosis factor
基因家族：Tumor necrosis factor superfamily
别名：TNFSF2; DIF; TNF-alpha
曾用名：TNFA
全称：TNF superfamily, member 2; tumor necrosis factor (TNF superfamily, member 2)

TNF 分子靶点综述

TNF-α 为单核细胞、巨噬细胞、中性粒细胞、NK 细胞和 CD4+T 细胞产生的 17 kDa 细胞因子。它在免疫应答中具有多种调节功能，还可作为潜在的热原。TNF-α对刺激物(感染因子或组织受损)产生应答后，在全身循环，激活中性粒细胞，改变血管内皮细胞的特性，调控其它组织的代谢活性，以及通过诱导局部凝血作用展现肿瘤杀伤活性。TNF-α 在关节组织和其它组织发生炎症的发病机制具有重要作用。

小鼠 Mouse Tnf 分子靶点信息

分子名：Tnf, tumor necrosis factor
别称：
- DIF
- TNF alpha
- TNF-alpha
- Tnfa
- TNFalpha
- Tnfsf1a
- tumor necrosis factor-alpha
- tumor necrosis factor, alpha
基因序列：NCBI_Gene: 21926
蛋白序列：

小鼠 Mouse Tnf 靶点分子功能（预测）

Enables cytokine activity; protease binding activity; and tumor necrosis factor receptor binding activity. Involved in several processes, including endothelial cell apoptotic process; positive regulation of cell communication; and positive regulation of macromolecule metabolic process. Acts upstream of or within several processes, including apoptotic signaling pathway; positive regulation of intracellular signal transduction; and regulation of gene expression. Located in several cellular components, including external side of plasma membrane; phagocytic cup; and recycling endosome. Is expressed in several structures, including alimentary system; brain; integumental system; lung; and reproductive system. Used to study several diseases, including autoimmune disease (multiple); congestive heart failure; heart valve disease (multiple); idiopathic pulmonary fibrosis; and spondyloarthropathy. Human ortholog(s) of this gene implicated in several diseases, including artery disease (multiple); autoimmune disease (multiple); eye disease (multiple); lung disease (multiple); and skin disease (multiple). Orthologous to human TNF (tumor necrosis factor).