EK361

Rat CCL3/MIP-1a ELISA Kit检测试剂盒（酶联免疫吸附法）

¥1,600.00 – ¥2,650.00

因产品会迭代升级，具体实验步骤请按纸质版说明书操作

说明书

在售SKU:70-EK361-48, 70-EK361-96, EK361

分子靶点：CCL3
种属：大鼠
样本类型：血清、血浆、细胞培养上清及其他生物学样本
检测样本体积：血清、血浆样本20μL
灵敏度：0.09pg/mL
检测范围：6.25-400pg/mL
回收率：90%-119%

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Size
库存号

ELISA试剂盒详细信息

商品名	Rat CCL3/MIP-1a ELISA Kit检测试剂盒（酶联免疫吸附法）
种属	大鼠
靶点	CCL3
检测方法	双抗体夹心法
检测样本类型	血清、血浆、细胞培养上清及其他生物学样本
检测样本体积	血清、血浆样本20μL
灵敏度	0.09pg/mL
线性范围	6.25-400pg/mL
精密度	板内变异系数：4.8%-5.0%;板间变异系数：3.5%-4.6%
回收率	90%-119%
平均回收率	105%
板式	96孔板，可拆
保存条件	2-8℃保存。已拆开：标准品-20℃保存，其它4℃。
运输条件	2-8℃冰袋运输
检测原理	本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗大鼠CCL3抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体，经过孵育，样本中存在的CCL3与固相抗体和检测抗体结合。洗涤去除未结合的物质后，加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后，加入显色底物TMB，避光显色。颜色反应的深浅与样本中CCL3的浓度成正比。加入终止液终止反应，在450nm波长(参考波长570 - 630nm)测定吸光度值。

文章目录[隐藏]

分子信息
CCL3 分子靶点信息概述
CCL3 分子靶点综述
- 大鼠 Rat Ccl3 分子靶点信息
- 大鼠 Rat Ccl3 靶点分子功能（预测）

分子信息

概述大鼠 CCL3, MIP-1-alpha 靶点信息

CCL3 分子靶点信息概述

分子名：CCL3, C-C motif chemokine ligand 3
基因家族：Chemokine ligands
别名：G0S19-1; LD78ALPHA; MIP-1-alpha
曾用名：SCYA3
全称：small inducible cytokine A3 (homologous to mouse Mip-1a); chemokine (C-C motif) ligand 3

CCL3 分子靶点综述

趋化因子配体3(CCL3)，又名巨噬细胞炎性蛋白1α(MIP-1α)，是CC趋化因子家族中的一种细胞因子，参与多形核白细胞招募和活化中的急性炎症状态。CCL3基因位于人类17号染色体上，其cDNAs编码92个氨基酸残基前体蛋白，该前体蛋白含有一段22个氨基酸残基信号肽，裂解该前体蛋白可产生分泌性的成熟蛋白。和其它趋化因子一样，CCL3是单核细胞趋化物。另外，有报道认为CCL3对T淋巴细胞、NK细胞、细胞毒性T细胞、B细胞、嗜碱性粒细胞和嗜酸性粒细胞有不同的趋化和促粘附作用。CCL3已被确定为是一种干细胞抑制剂(SCI)，可抑制造血祖细胞在体外和体内的增殖。CCL3、CCL4和RANTES被认为是由CD8+T细胞产生的重要的HIV抑制因子。

大鼠 Rat Ccl3 分子靶点信息

分子名：Ccl3, C-C motif chemokine ligand 3
别称：
- C-C motif chemokine 3
- chemokine (C-C motif) ligand 3
- macrophage inflammatory protein 1 alpha (Small inducible cytokine A3)
- macrophage inflammatory protein 1-alpha
- MIP-1-alpha
- MIP-1a
- Scya3
- small inducible cytokine A3
- small-inducible cytokine A3
基因序列：NCBI_Gene: 25542
蛋白序列：UniProtKB: P50229

大鼠 Rat Ccl3 靶点分子功能（预测）

Enables chemokine activity. Involved in several processes, including neutrophil chemotaxis; positive regulation of cytosolic calcium ion concentration; and positive regulation of osteoclast differentiation. Predicted to be located in cytosol and extracellular region. Predicted to be active in extracellular space. Biomarker of several diseases, including Chagas disease; anterior uveitis; nephritis (multiple); renal fibrosis; and status epilepticus. Human ortholog(s) of this gene implicated in human immunodeficiency virus infectious disease. Orthologous to several human genes including CCL3 (C-C motif chemokine ligand 3).