EK318

Rat IL-18 ELISA Kit检测试剂盒（酶联免疫吸附法）

Name: Rat IL-18 ELISA Kit检测试剂盒（酶联免疫吸附法）
SKU: EK318
Availability: InStock

¥1,600.00 – ¥2,650.00

因产品会迭代升级，具体实验步骤请按纸质版说明书操作

在售SKU:70-EK318-48, 70-EK318-96, EK318, il-18

分子靶点：IL18
种属：大鼠
样本类型：血清、血浆样本
检测样本体积：血清、血浆样本10μL
灵敏度：1.81pg/mL
检测范围：15.63-1000pg/mL
回收率：86%-119%

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Size
库存号

ELISA试剂盒详细信息

商品名	Rat IL-18 ELISA Kit检测试剂盒（酶联免疫吸附法）
种属	大鼠
靶点	IL18
检测方法	双抗体夹心法
检测样本类型	血清、血浆样本
检测样本体积	血清、血浆样本10μL
灵敏度	1.81pg/mL
线性范围	15.63-1000pg/mL
精密度	板内变异系数：3.21%-6.34%;板间变异系数：2.6-5.12%
回收率	86%-119%
平均回收率	100%
板式
保存条件	2-8℃保存。已拆开：标准品-20℃保存，其它4℃。
运输条件	2-8℃冰袋运输
检测原理	本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性捕获抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体，经过孵育，样本中存在的待测物质与捕获抗体和检测抗体结合。洗涤去除未结合的物质后，加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后，加入显色底物（TMB），避光显色。颜色反应的深浅与样本中待测物质的浓度成正比。加入终止液终止反应，在450nm波长(参考波长570 - 630nm)测定吸光度值。

文章目录[隐藏]

分子信息
IL18 分子靶点信息概述
IL18 分子靶点综述
- 大鼠 Mouse Il18 分子靶点信息
- 大鼠 Rat Il18 靶点分子功能（预测）

分子信息

概述大鼠 IL18, IGIF, IL1F4 靶点信息

IL18 分子靶点信息概述

分子名：IL18, interleukin 18
基因家族：Interleukins
别名：IGIF; IL1F4; IL-1g; IL-18
全称：interferon-gamma-inducing factor; interleukin 18 (interferon-gamma-inducing factor)

IL18 分子靶点综述

白细胞介素18(IL-18)，又名干扰素γ诱导因子，是一种促炎性细胞因子，在人类中由IL18基因编码。IL-18属于IL-1超家族，由巨噬细胞和其它细胞分泌产生。IL-18刺激后，自然杀伤细胞(NK)和某些T细胞就会释放另一种重要的细胞因子—干扰素γ或Ⅱ型干扰素，进而激活巨噬细胞或其它细胞。IL-18可诱导产生严重的炎症反应，同样可增加人类神经元中β-淀粉样蛋白(与阿尔茨海默氏病相关)的产生。

大鼠 Mouse Il18 分子靶点信息

分子名：Il18, interleukin 18
别称：
- IFN-gamma-inducing factor
- IL-1 gamma
- IL-18
- interferon gamma-inducing factor
- interferon-gamma-inducing factor
- interleukin-1 gamma
- interleukin-18
基因序列：NCBI_Gene: 29197
蛋白序列：
- UniprotKB: P97636
- UniprotKB: Z4YNI9

大鼠 Rat Il18 靶点分子功能（预测）

Predicted to enable cytokine activity and interleukin-18 receptor binding activity. Involved in several processes, including cellular response to hormone stimulus; positive regulation of cell population proliferation; and positive regulation of cytokine production. Located in apical plasma membrane; cytoplasm; and extracellular space. Used to study brain edema; fatty liver disease; heart disease; status epilepticus; and vasculitis. Biomarker of several diseases, including cardiac arrest; ischemia (multiple); lung disease (multiple); perinatal necrotizing enterocolitis; and urinary tract infection. Human ortholog(s) of this gene implicated in several diseases, including Kawasaki disease; allergic rhinitis; autoimmune disease (multiple); biliary atresia; and hepatitis B. Orthologous to human IL18 (interleukin 18).