EK171EGA

EasyGo!™ Human IgG One Step ELISA Kit 检测试剂盒（酶联免疫吸附法）

Name: EasyGo!™ Human IgG One Step ELISA Kit 检测试剂盒（酶联免疫吸附法）
SKU: EK171EGA
Availability: InStock

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因产品会迭代升级，具体实验步骤请按纸质版说明书操作

在售SKU:70-EK171EGA-48, 70-EK171EGA-96, EK171EGA

分子靶点：IgG
种属：人
样本类型：人血清、血浆
检测样本体积：血清、血浆样本5μL
灵敏度：0.04ng/mL
检测范围：1.56-100ng/mL
回收率：77%-122%

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ELISA试剂盒详细信息

商品名	EasyGo!™ Human IgG One Step ELISA Kit 检测试剂盒（酶联免疫吸附法）
种属	人
靶点	IgG
检测方法	双抗体夹心法
检测样本类型	人血清、血浆
检测样本体积	血清、血浆样本5μL
灵敏度	0.04ng/mL
线性范围	1.56-100ng/mL
精密度	板内变异系数：8.1%-9.0%;板间变异系数：5.8%-7.8%
回收率	77%-122%
板式	96孔板，可拆
保存条件	2-8℃保存。已拆开：标准品-20℃保存，其它4℃。
运输条件	2-8℃冰袋运输
检测原理	本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性捕获抗体预包被在高亲和力的酶标板上，酶标板孔中预先加入辣根过氧化物酶标记的检测抗体微球。酶标板孔中加入标准品和待测样本，经过孵育，样本中存在的待测物质与捕获抗体和检测抗体结合。洗涤去除未结合的物质后，加入显色底物（TMB），避光显色。颜色反应的深浅与样本中待测物质的浓度成正比。加入终止液终止反应，在450nm波长 (参考波长570 - 630nm) 测定吸光度值。