EK115

Human IL-15 ELISA Kit检测试剂盒（酶联免疫吸附法）

¥1,600.00 – ¥2,650.00

因产品会迭代升级，具体实验步骤请按纸质版说明书操作

说明书

在售SKU:70-EK115-48, 70-EK115-96, EK115

分子靶点：IL15
种属：人
样本类型：血清、血浆、细胞培养上清及其他生物学样本
检测样本体积：血清、血浆样本50μL
灵敏度：0.6pg/mL
检测范围：3.13-200pg/mL
回收率：80%-98%

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Size
库存号

ELISA试剂盒详细信息

商品名	Human IL-15 ELISA Kit检测试剂盒（酶联免疫吸附法）
种属	人
靶点	IL15
检测方法	双抗体夹心法
检测样本类型	血清、血浆、细胞培养上清及其他生物学样本
检测样本体积	血清、血浆样本50μL
灵敏度	0.6pg/mL
线性范围	3.13-200pg/mL
精密度	板内变异系数：7.1%-9%;板间变异系数：7.8%-9.5%
回收率	80%-98%
平均回收率	88%
板式	96孔板，可拆
保存条件	2-8℃保存。已拆开：标准品-20℃保存，其它4℃。
运输条件	2-8℃冰袋运输
检测原理	本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人IL-15抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体，经过孵育，样本中存在的IL-15与固相抗体和检测抗体结合。洗涤去除未结合的物质后，加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后，加入显色底物TMB，避光显色。颜色反应的深浅与样本中IL-15的浓度成正比。加入终止液终止反应，在450nm波长(参考波长570 - 630nm)测定吸光度值。

文章目录[隐藏]

分子信息
IL15 分子靶点信息概述
IL15 分子靶点综述
- 人 Human IL15 分子靶点信息
- 人 Human IL15靶点分子功能（预测）

分子信息

概述人 IL15 靶点信息

IL15 分子靶点信息概述

分子名：IL15, interleukin 15
基因家族：Interleukins
别名：IL-15; MGC9721

IL15 分子靶点综述

白细胞介素15(IL-15)是一种促炎性蛋白，在中性粒细胞、树突状细胞和巨噬细胞的活化中起作用，对于NK细胞和CD8+T细胞的发育和生存是必不可少的。IL-15通过异三聚体受体信号激活Jak/Stat途径。虽然IL-15mRNA在许多细胞类型中表达，但是该分泌蛋白在生物样品中很少检测得到。最近的研究表明，IL-15滞留在胞内，只有当与其唯一的受体IL-15Rα形成复合物才能分泌到胞外。可溶性IL-15/IL-15R复合物由小鼠树突状细胞、成纤维细胞和巨噬细胞产生，它的结合活性比单独的IL-15提高10-100倍。

人 Human IL15 分子靶点信息

分子名：IL15, interleukin 15
别称：
- IL-15
- interleukin-15
- MGC9721
基因序列：NCBI_Gene: 3600
蛋白序列：UniProtKB: P40933

人 Human IL15靶点分子功能（预测）

Predicted to enable cytokine activity. Involved in several processes, including cell surface receptor signaling pathway; macrophage differentiation; and positive regulation of macromolecule metabolic process. Located in cytosol and nuclear speck. Implicated in aspergillosis; asthma; pleurisy; and type 1 diabetes mellitus. Biomarker of several diseases, including artery disease (multiple); brain infarction; lung disease (multiple); obesity; and viral infectious disease (multiple).