EK1147

Human RBP4 ELISA Kit检测试剂盒(酶联免疫吸附法)

¥1,600.00¥2,650.00

因产品会迭代升级,具体实验步骤请按纸质版说明书操作

在售SKU:70-EK1147-48, 70-EK1147-96, EK1147

  • 分子靶点:RBP4
  • 种属:
  • 样本类型:血清、血浆、细胞培养上清及其他生物学样本
  • 检测样本体积:血清、血浆样本10μL
  • 灵敏度:1.05pg/mL
  • 检测范围:31.25-2000pg/mL
  • 回收率:88%-110%
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ELISA试剂盒详细信息

商品名Human RBP4 ELISA Kit检测试剂盒(酶联免疫吸附法)
种属
靶点RBP4
检测方法双抗体夹心法
检测样本类型血清、血浆、细胞培养上清及其他生物学样本
检测样本体积血清、血浆样本10μL
灵敏度1.05pg/mL
线性范围31.25-2000pg/mL
精密度板内变异系数:3.6%-5.9%;板间变异系数:3.2%-4.4%
回收率88%-110%
平均回收率102%
板式96孔板,可拆
保存条件2-8℃保存。已拆开:标准品-20℃保存,其它4℃。
运输条件2-8℃冰袋运输
检测原理本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人RBP4抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体,经过孵育,样本中存在的RBP4与固相抗体和检测抗体结合。洗涤去除未结合的物质后,加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后,加入显色底物TMB,避光显色。颜色反应的深浅与样本中RBP4的浓度成正比。加入终止液终止反应,在450nm波长(参考波长570 - 630nm)测定吸光度值。

分子信息

概述人 RBP4 靶点信息

RBP4 分子靶点信息概述

  • 分子名:RBP4, retinol binding protein 4
  • 基因家族:Lipocalins
  • 全称:retinol-binding protein 4, plasma

RBP4 分子靶点综述

视黄醇结合蛋白4(RBP4)属于载脂蛋白家族,是血液中视黄醇(维生素A醇)特异性的载体。它可将视黄醇从肝脏运输至外周组织。最近,RBP4被认为是一种脂肪因子,在AG4KO小鼠中有助于对胰岛素耐受。它由脂肪细胞分泌,当血糖浓度下降时,可对其它细胞传递信号。RBP4基因突变与常染色体显性小眼畸形、无眼和眼组织残缺有关。

人 Human RBP4 分子靶点信息

  • 分子名:RBP4, retinol binding protein 4
  • 别称:
    • MCOPCB10
    • plasma retinol-binding protein
    • PRBP
    • RBP
    • RDCCAS
    • retinol binding protein 4, plasma
    • retinol-binding protein 4
    • retinol-binding protein 4, interstitial
  • 基因序列:NCBI_Gene: 5950
  • 蛋白序列:UniProtKB: P02753

人 Human RBP4靶点分子功能(预测)

Enables retinol binding activity. Involved in several processes, including gluconeogenesis; positive regulation of insulin secretion; and retinol metabolic process. Located in extracellular space. Implicated in type 2 diabetes mellitus. Biomarker of type 2 diabetes mellitus.

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