EK182

Human TNF-a ELISA Kit检测试剂盒（酶联免疫吸附法）

¥1,600.00 – ¥10,800.00

因产品会迭代升级，具体实验步骤请按纸质版说明书操作

说明书

在售SKU:70-EK182-48, 70-EK182-96, EK182

分子靶点：TNF
种属：人
样本类型：血清，血浆，细胞培养上清及其他生物学样本
检测样本体积：血清、血浆样本50 μL
灵敏度：5.77pg/mL
检测范围：15.63-1000pg/mL
回收率：89%-120%

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Size
库存号

ELISA试剂盒详细信息

商品名	Human TNF-a ELISA Kit检测试剂盒（酶联免疫吸附法）
种属	人
靶点	TNF
检测方法	双抗体夹心法
检测样本类型	血清，血浆，细胞培养上清及其他生物学样本
检测样本体积	血清、血浆样本50 μL
灵敏度	5.77pg/mL
线性范围	15.63-1000pg/mL
精密度	板内变异系数：3.8%-8.9%;板间变异系数：5.0%-8.4%
回收率	89%-120%
平均回收率	103%
板式	96孔板，可拆
保存条件	2-8℃保存。已拆开：标准品-20℃保存，其它4℃。
运输条件	2-8℃冰袋运输
检测原理	本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性捕获抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体，经过孵育，样本中存在的待测物质与捕获抗体和检测抗体结合。洗涤去除未结合的物质后，加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后，加入显色底物（TMB），避光显色。颜色反应的深浅与样本中待测物质的浓度成正比。加入终止液终止反应，在450nm波长(参考波长570 - 630nm)测定吸光度值。

文章目录[隐藏]

分子信息
TNF 分子靶点信息概述
TNF 分子靶点综述
- 人 Human TNF 分子靶点信息
- 人 Human TNF靶点分子功能（预测）

分子信息

概述人 TNF, TNFA, TNFSF2 靶点信息

TNF 分子靶点信息概述

分子名：TNF, tumor necrosis factor
基因家族：Tumor necrosis factor superfamily
别名：TNFSF2; DIF; TNF-alpha
曾用名：TNFA
全称：TNF superfamily, member 2; tumor necrosis factor (TNF superfamily, member 2)

TNF 分子靶点综述

TNF-α 为单核细胞、巨噬细胞、中性粒细胞、NK 细胞和 CD4+T 细胞产生的 17 kDa 细胞因子。它在免疫应答中具有多种调节功能，还可作为潜在的热原。TNF-α对刺激物(感染因子或组织受损)产生应答后，在全身循环，激活中性粒细胞，改变血管内皮细胞的特性，调控其它组织的代谢活性，以及通过诱导局部凝血作用展现肿瘤杀伤活性。TNF-α 在关节组织和其它组织发生炎症的发病机制具有重要作用。

人 Human TNF 分子靶点信息

分子名：TNF, tumor necrosis factor
别称：
- APC1 protein
- cachectin
- DIF
- TNF superfamily, member 2
- TNF-a
- TNF-alpha
- TNF, macrophage-derived
- TNF, monocyte-derived
- TNFA
- TNFSF2
- TNLG1F
- tumor necrosis factor alpha
- tumor necrosis factor ligand 1F
- tumor necrosis factor ligand superfamily member 2
- tumor necrosis factor-alpha
基因序列：NCBI_Gene: 7124
蛋白序列：UniProtKB: P01375

人 Human TNF靶点分子功能（预测）

Enables several functions, including identical protein binding activity; protease binding activity; and signaling receptor binding activity. Involved in several processes, including negative regulation of macromolecule metabolic process; positive regulation of macromolecule metabolic process; and regulation of apoptotic process. Acts upstream of or within several processes, including regulation of cellular protein metabolic process; regulation of gene expression; and regulation of signal transduction. Located in cell surface; extracellular space; and membrane raft. Is integral component of plasma membrane. Implicated in several diseases, including artery disease (multiple); autoimmune disease (multiple); eye disease (multiple); lung disease (multiple); and skin disease (multiple). Biomarker of several diseases, including anemia (multiple); autoimmune disease (multiple); eye disease (multiple); kidney disease (multiple); and lung disease (multiple).