EK2173

Mouse Granzyme B ELISA Kit检测试剂盒（酶联免疫吸附法）

¥1,600.00 – ¥2,650.00

因产品会迭代升级，具体实验步骤请按纸质版说明书操作

说明书

在售SKU:70-EK2173-48, 70-EK2173-96, EK2173

分子靶点：GZMB
种属：小鼠
样本类型：血清、血浆、细胞培养上清及其他生物学样本
检测样本体积：血清、血浆样本10μL
灵敏度：9.91pg/mL
检测范围：0.31-20ng/mL
回收率：91%-105%

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Size
库存号

ELISA试剂盒详细信息

商品名	Mouse Granzyme B ELISA Kit检测试剂盒（酶联免疫吸附法）
种属	小鼠
靶点	GZMB
检测方法	双抗体夹心法
检测样本类型	血清、血浆、细胞培养上清及其他生物学样本
检测样本体积	血清、血浆样本10μL
灵敏度	9.91pg/mL
线性范围	0.31-20ng/mL
精密度	板内变异系数：2.9%-3.9%;板间变异系数：1.8%-2.8%
回收率	91%-105%
平均回收率	99%
板式	96孔板，可拆
保存条件	2-8℃保存。已拆开：标准品-20℃保存，其它4℃。
运输条件	2-8℃冰袋运输
检测原理	本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗小鼠GranzymeB抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体，经过孵育，样本中存在的GranzymeB与固相抗体和检测抗体结合。洗涤去除未结合的物质后，加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后，加入显色底物TMB，避光显色。颜色反应的深浅与样本中GranzymeB的浓度成正比。加入终止液终止反应，在450nm波长(参考波长570 - 630nm)测定吸光度值。

文章目录[隐藏]

分子信息
GZMB 分子靶点信息概述
GZMB 分子靶点综述
- 小鼠 Mouse Gzme 分子靶点信息
- 小鼠 Mouse Gzme 靶点分子功能（预测）

分子信息

概述小鼠 GZMB, granzyme B, CTLA1 靶点信息

GZMB 分子靶点信息概述

分子名：GZMB, granzyme B
基因家族：Granule associated serine proteases of immune defence
别名：CCPI; CGL-1; CSP-B; CGL1; CTSGL1; HLP; SECT
曾用名：CTLA1; CSPB
全称：fragmentin 2; cytotoxic serine protease B; cathepsin G-like 1; T-cell serine protease 1-3E; granzyme 2; cytotoxic T-lymphocyte-associated serine esterase 1; granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1)

GZMB 分子靶点综述

颗粒酶B(GranzymeB)是一种丝氨酸蛋白酶，最常见于细胞毒性淋巴细胞(CTLs)和自然杀伤细胞(NK细胞)颗粒中。由这些细胞分泌的GranzymeB和穿孔蛋白一起作用介导靶细胞的凋亡。最近，人们发现许多非细胞毒性细胞从嗜碱性粒细胞、肥大细胞到平滑肌细胞也可产生GranzymeB。GranzymeB通过刺激细胞因子的释放参与诱导炎症，同样也参与细胞外基质重塑。升高的GranzymeB水平与许多自身免疫性疾病、一些皮肤病和1型糖尿病有关。

小鼠 Mouse Gzme 分子靶点信息

分子名：Gzme, granzyme E
别称：
- CCP3
- Ctla-6
- Ctla6
- cytotoxic T lymphocyte-associated protein 6
- MCSP-2
基因序列：NCBI_Gene: 14942
蛋白序列：
- UniprotKB: P08884

小鼠 Mouse Gzme 靶点分子功能（预测）

Predicted to enable serine-type endopeptidase activity. Predicted to be involved in several processes, including granzyme-mediated programmed cell death signaling pathway; natural killer cell mediated cytotoxicity; and pyroptosis. Predicted to act upstream of or within cytolysis. Predicted to be located in cytolytic granule. Predicted to be active in cytoplasm and intracellular membrane-bounded organelle. Is expressed in extraembryonic component; placenta; spongiotrophoblast; and trophoblast giant cell. Orthologous to human GZMB (granzyme B).