EK8103

Prostaglandin E2/PGE2 Competitive ELISA Kit 检测试剂盒（酶联免疫吸附法）

¥2,500.00 – ¥4,000.00

因产品会迭代升级，具体实验步骤请按纸质版说明书操作

说明书

在售SKU:70-EK8103/2-48, 70-EK8103/2-96, EK8103

种属：人、小鼠、大鼠
样本类型：血清、血浆、细胞培养上清及其他生物学样本
检测样本体积：血清、血浆样本5μL
灵敏度：1.08pg/mL
检测范围：31,25-2000pg/ml
回收率：92%-102%

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Size
库存号

ELISA试剂盒详细信息

商品名	Prostaglandin E2/PGE2 Competitive ELISA Kit 检测试剂盒（酶联免疫吸附法）
种属	人、小鼠、大鼠
检测方法	双抗体夹心法
检测样本类型	血清、血浆、细胞培养上清及其他生物学样本
检测样本体积	血清、血浆样本5μL
灵敏度	1.08pg/mL
线性范围	31,25-2000pg/ml
精密度	板内变异系数：3.9%-4.3%;板间变异系数：5.2%-6.5%
回收率	92%-102%
平均回收率	99%
板式	96孔板，可拆
保存条件	组分A：2-8°C，组分B：-20°C；详见说明书。
运输条件	组分A、B分开运输
检测原理	本试剂盒采用固相竞争酶联免疫吸附检测技术。兔抗小鼠抗体预包被在高亲和力的酶标板上。PGE2特异性的单克隆抗体加入至孔中，经过孵育，与固相抗体结合。洗涤后，加入生物素标记的PGE2和未标记的PGE2或样本，竞争结合单克隆抗体上有限的结合位点。洗涤去除未结合的物质后，加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后，加入显色底物，颜色反应的深浅与PGE2的浓度成反比。加入终止液终止反应，在450 nm波长(参考波长570 - 630 nm)测定吸光度值。本试剂盒已验证人、小鼠和大鼠血清样本，但其它哺乳动物的PGE2预计也可被检测。