EK301A

Rat IL-1α ELISA Kit检测试剂盒（酶联免疫吸附法）

¥1,600.00 – ¥2,650.00

因产品会迭代升级，具体实验步骤请按纸质版说明书操作

说明书

在售SKU:70-EK301A/2-48, 70-EK301A/2-96, EK301A

分子靶点：IL1A
种属：大鼠
样本类型：血清、血浆、细胞培养上清及其他生物学样本
检测样本体积：血清、血浆样本20μL
灵敏度：0.25pg/mL
检测范围：7.81-500pg/mL
回收率：72%-129%

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Size
库存号

ELISA试剂盒详细信息

商品名	Rat IL-1α ELISA Kit检测试剂盒（酶联免疫吸附法）
种属	大鼠
靶点	IL1A
检测方法	双抗体夹心法
检测样本类型	血清、血浆、细胞培养上清及其他生物学样本
检测样本体积	血清、血浆样本20μL
灵敏度	0.25pg/mL
线性范围	7.81-500pg/mL
精密度	板内变异系数：4.6%-5.8%;板间变异系数：1.7%-6.5%
回收率	72%-129%
平均回收率	103%
板式	96孔板，可拆
保存条件	2-8℃保存。已拆开：标准品-20℃保存，其它4℃。
运输条件	2-8℃冰袋运输
检测原理	本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗大鼠IL-1α抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体，经过孵育，样本中存在的IL-1α与固相抗体和检测抗体结合。洗涤去除未结合的物质后，加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后，加入显色底物TMB，避光显色。颜色反应的深浅与样本中IL-1α的浓度成正比。加入终止液终止反应，在450nm波长(参考波长570 - 630nm)测定吸光度值。

文章目录[隐藏]

分子信息
IL1A 分子靶点信息概述
IL1A 分子靶点综述
- 大鼠 Rat Il1a 分子靶点信息
- 大鼠 Rat Il1a 靶点分子功能（预测）

分子信息

概述大鼠 IL1A, IL1-α, IL1F1 靶点信息

IL1A 分子靶点信息概述

分子名：IL1A, interleukin 1 alpha
基因家族：Interleukins
别名：IL1F1; IL-1A; IL1-ALPHA
曾用名：IL1
全称：preinterleukin 1 alpha; hematopoietin-1; pro-interleukin-1-alpha

IL1A 分子靶点综述

白细胞介素1(IL-1)是17kDa的胞外多肽，分为IL-1α和IL-1β两种蛋白。IL-1α是一种多效性细胞因子，在人类中由IL1A基因编码。IL-1α主要由活化的巨噬细胞、中性粒细胞、上皮细胞和内皮细胞产生。它具有代谢、生理、造血活性，在调节免疫应答中起到核心作用。IL-1α可与HAX1、NDN相互作用，但是与TNF之间的协同作用是临床上最相关的。IL-1α和TNF都是急性期细胞因子，具有促发热和发炎作用。人们目前正在评估IL-1α是否可作为临床试验中一个潜在治疗肿瘤的标志物。阻断IL-1α活性具有潜在治疗皮肤病的作用，例如痤疮。

大鼠 Rat Il1a 分子靶点信息

分子名：Il1a, interleukin 1 alpha
别称：
- IL-1 alpha
- IL-1F1
- interleukin-1 alpha
基因序列：NCBI_Gene: 24493
蛋白序列：

大鼠 Rat Il1a 靶点分子功能（预测）

Predicted to enable copper ion binding activity; cytokine activity; and interleukin-1 receptor binding activity. Involved in several processes, including keratinization; negative regulation of establishment of Sertoli cell barrier; and positive regulation of intracellular signal transduction. Located in several cellular components, including cell surface; cytosol; and extracellular space. Used to study interstitial lung disease. Biomarker of several diseases, including endometritis; lung disease (multiple); urinary tract infection; uveitis; and varicocele. Human ortholog(s) of this gene implicated in several diseases, including Fabry disease; Schnitzler syndrome; autoimmune disease (multiple); hematologic cancer (multiple); and lung disease (multiple). Orthologous to human IL1A (interleukin 1 alpha).