EK165

Human TSLP ELISA Kit检测试剂盒（酶联免疫吸附法）

¥1,600.00 – ¥2,650.00

因产品会迭代升级，具体实验步骤请按纸质版说明书操作

说明书

在售SKU:70-EK165-48, 70-EK165-96, EK165

分子靶点：TSLP
种属：人
样本类型：血清、血浆、细胞培养上清及其他生物学样本
检测样本体积：血清、血浆样本20μL
灵敏度：0.13pg/mL
检测范围：15.63-1000pg/mL
回收率：85%-118%

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Size
库存号

ELISA试剂盒详细信息

商品名	Human TSLP ELISA Kit检测试剂盒（酶联免疫吸附法）
种属	人
靶点	TSLP
检测方法	双抗体夹心法
检测样本类型	血清、血浆、细胞培养上清及其他生物学样本
检测样本体积	血清、血浆样本20μL
灵敏度	0.13pg/mL
线性范围	15.63-1000pg/mL
精密度	板内变异系数：3.3%-4.1%;板间变异系数：5.2%-7.5%
回收率	85%-118%
平均回收率	100%
板式	96孔板，可拆
保存条件	2-8℃保存。已拆开：标准品-20℃保存，其它4℃。
运输条件	2-8℃冰袋运输
检测原理	本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人TSLP抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品和待测样本，经过孵育，样本中存在的TSLP与固相抗体结合。洗涤去除未结合的物质后，加入生物素化的检测抗体孵育。洗涤去除未结合的生物素化的抗体，加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后，加入显色底物TMB，避光显色。颜色反应的深浅与样本中TSLP的浓度成正比。加入终止液终止反应，在450nm波长(参考波长570 - 630nm)测定吸光度值。

文章目录[隐藏]

分子信息
TSLP 分子靶点信息概述
TSLP 分子靶点综述
- 人 Human TSLP 分子靶点信息
- 人 Human TSLP靶点分子功能（预测）

分子信息

概述人 TSLP 靶点信息

TSLP 分子靶点信息概述

分子名：TSLP, thymic stromal lymphopoietin

TSLP 分子靶点综述

胸腺基质淋巴细胞生成素(TSLP)主要由非造血细胞如成纤维细胞、上皮细胞和不同类型的基质或基质样细胞产生。它主要影响骨髓细胞、诱导单核细胞释放T细胞虏获趋化因子和增强髓样(CD11c+)树突状细胞成熟。研究表明，TSLP还可激活位于表皮的特定树突状细胞亚群—朗格汉斯细胞的成熟。胸腺TSLP激活髓样和浆细胞样(CD123+)树突状细胞会导致调节性T细胞的产生。TSLP通过胸腺基质淋巴细胞受体CRLF2和IL-7Rα链组成的异源二聚体受体复合物产生信号。结合STAT5后就会诱导其发生磷酸化，进而导致上游转录因子的表达。TSLP的表达与许多疾病包括哮喘、炎症性关节炎、特应性皮炎、湿疹及其它过敏状态有关。

人 Human TSLP 分子靶点信息

分子名：TSLP, thymic stromal lymphopoietin
别称：
- 无
基因序列：NCBI_Gene: 85480
蛋白序列：UniProtKB: Q969D9

人 Human TSLP靶点分子功能（预测）

Enables cytokine activity and interleukin-7 receptor binding activity. Involved in several processes, including defense response to other organism; positive regulation of cytokine production; and positive regulation of signal transduction. Located in extracellular region. Biomarker of common cold and respiratory syncytial virus infectious disease.