FC21201

小鼠炎症十二项细胞因子检测试剂盒

¥9,900.00 – ¥15,000.00

因产品会迭代升级，具体实验步骤请按纸质版说明书操作

说明书

TNF-α, TNF, tumor necrosis factor, Tumor necrosis factor superfamily, TNFSF2; DIF; TNF-alpha, TNFA, TNF superfamily, member 2; tumor necrosis factor (TNF superfamily, member 2), Tnf, tumor necrosis factor, , DIF, TNF alpha, TNF-alpha, Tnfa, TNFalpha, Tnfsf1a, tumor necrosis factor-alpha, tumor necrosis factor, alpha, IFN-γ, IFNG, interferon gamma, Ifng, interferon gamma, , Ifg, IFN-gamma, IL-1β, IL1B, interleukin 1 beta, Interleukins, IL1F2; IL-1B; IL1-BETA, IL1B, interleukin 1 beta, , catabolin, IL-1, IL-1 beta, IL1-BETA, IL1beta, IL1F2, interleukin 1, beta, interleukin 1beta, interleukin-1 beta, preinterleukin 1 beta, pro-interleukin-1-beta, IL-2, IL2, interleukin 2, Interleukins, IL-2; TCGF, T cell growth factor, Il2, interleukin 2, , IL-2, Il-2, IL-4, IL4, interleukin 4, Interleukins, BSF1; IL-4; BCGF1; BCGF-1; MGC79402, B_cell stimulatory factor 1; lymphocyte stimulatory factor 1; B cell growth factor 1, IL-5, IL5, interleukin 5, Interleukins, IL-5; EDF; TRF, interleukin-5; T-cell replacing factor; B cell differentiation factor I; eosinophil differentiation factor; colony-stimulating factor, eosinophil; interleukin 5 (colony-stimulating factor, eosinophil), IL-6, IL6, interleukin 6, Interferons; Interleukins; Interleukin 6 type cytokine family, IL-6; BSF2; HGF; HSF, IFNB2, interferon, beta 2; interleukin 6 (interferon, beta 2), Ifnb2, IL-6, ILg6, Interleukin 6 (interferon beta 2), Interleukin 6 (interferon, beta 2), interleukin-6, IL-10, IL10, interleukin 10, Interleukins, CSIF; TGIF; IL10A; IL-10, cytokine synthesis inhibitory factor; T-cell growth inhibitory factor, Il10, interleukin 10, , cytokine synthesis inhibitory factor, IL-10, IL-12, IL12A, interleukin 12A, Interleukins, CLMF; IL-12A; p35; NFSK, NKSF1, nterleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35); natural killer cell stimulatory factor 1, 35 kD subunit; cytotoxic lymphocyte maturation factor 1, p35; interleukin 12, p35; IL-12, subunit p35; NF cell stimulatory factor chain 1; interleukin-12 alpha chain; IL35 subunit, Il12a, interleukin 12a, , Il-12a, IL-12p35, p35, IL-17A, IL17A, interleukin 17A, Interleukins, IL-17A; IL-17, CTLA8; IL17, cytotoxic T-lymphocyte-associated protein 8; interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8), Il17a, interleukin 17A, , Ctla-8, Ctla8, cytotoxic T lymphocyte-associated protein 8, IL-17A, Il17, interleukin 17, GM-CSF, CSF2, colony stimulating factor 2, GM-CSF; GMCSF, sargramostim; molgramostim; granulocyte-macrophage colony stimulating factor; colony stimulating factor 2 (granulocyte-macrophage), Csf2, colony stimulating factor 2 (granulocyte-macrophage), , colony stimulating factor, granulocyte macrophage, Csfgm, Gm-CSf, GMCSF, MGI-IGM, MCP-1, CCL2, C-C motif chemokine ligand 2, Chemokine ligands, MCP1; MCP-1; MCAF; SMC-CF; GDCF-2; HC11; MGC9434, SCYA2, monocyte chemotactic protein 1, homologous to mouse Sig-je; monocyte chemoattractant protein-1; monocyte chemotactic and activating factor; monocyte secretory protein JE; small inducible cytokine subfamily A (Cys-Cys), member 2; small inducible cytokine A2 (monocyte chemotactic protein 1, homologous to mouse Sig-je); chemokine (C-C motif) ligand 2, Ccl2, chemokine (C-C motif) ligand 2, , AI323594, expressed sequence AI323594, HC11, MCAF, MCP-1, MCP1, monocyte chemoattractant protein-1, monocyte chemotactic protein, Scya2, Sigje, small inducible cytokine A2, small inducible gene JE, SMC-CF, FC21201, FC21201-96, FC21201-48

分子靶点：CCL2、CSF2、IFNG、IL10、IL12、IL17A、IL1B、IL2、IL4、IL5、IL6、TNF

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Size
库存号

Multi-Plex 多因子检测试剂盒详细信息

产品名	小鼠炎症十二项细胞因子检测试剂盒
英文名	Mouse Inflammatory 12-Plex Assay Kit
种属	小鼠 (Mouse)
靶点	CCL2 CSF2 IFNG IL10 IL12 IL17A IL1B IL2 IL4 IL5 IL6 TNF
检测时间	3h
检测样本类型	血清或血浆
检测样本体积	25μL
荧光标记	APCPEAPC-Cy7
检测仪器	流式细胞仪
存储条件	试剂盒保存在 2-8℃，应避光保存，禁止冷冻
有效期	未开封 12 个月，开封后储存在 2 - 8℃ 可稳定 1 个月
检测原理	本试剂盒利用液相芯片与双抗夹心相结合的原理。采用两种不同尺寸（4μm和5μm）、十二种不同荧光强度的微球群上分别包被 IL-1β、IL-2、IL-4、IL-5、IL-6、GM-CSF、IL-10、IL-12p70、IL-17A、TNF-α、MCP-1、IFN-γ特异性抗体（捕获微球），可与样本中对应抗原蛋白特异性结合，加入生物素标记的检测抗体，形成抗体捕获微球-细胞因子-检测抗体的免疫复合物；洗涤去除多余的未结合抗体后再加入链霉亲和素-藻红蛋白偶联物（SA-PE），在流式细胞仪上检测相应荧光。通过免疫复合物的荧光强度得到待测样本IL-1β、IL-2、IL-4、IL-5、IL-6、GM-CSF、IL-10、IL-12p70、IL-17A、TNF-α、MCP-1、IFN-γ的含量。